血清可溶性CD30在电离辐射剂量估算中的价值  

Value of serum soluble CD30 as a biomarker for radiation exposure

作　　者：刘琼[1] 何颖[1] 沈先荣[1] 侯登勇[1] 刘玉明[1] 陈伟[1] 蒋定文[1] 王庆蓉[1] 李珂娴[1] 

机构地区：[1]海军医学研究所防护医学研究室,上海200433

出　　处：《解放军医学杂志》2016年第2期158-161,共4页Medical Journal of Chinese People's Liberation Army

基　　金：上海市科学技术委员会医学引导类科研项目(10411969300);上海市科学技术委员会自然科学基金(13ZR1418500);全军后勤科研项目(AHJ09J012);上海市卫生局科研计划课题(20124435)~~

摘　　要：目的探讨血清可溶性CD 3 0(s CD 3 0)作为电离辐射剂量估算的生物指标的可行性。方法健康雄性C57BL/6小鼠300只,随机分为对照组及1、3、5、7Gy辐照组(n=60)。辐照组小鼠接受60Coγ射线一次性全身辐照,剂量率为0.78Gy/min。各组分别于辐照后24、48、72h留取血清样本(每组每时间点20只小鼠),采用定量抗体芯片检测s CD30浓度,并通过回归分析法分析血清s CD30浓度与辐照剂量的相关性。结果未辐照小鼠s CD30正常值范围为121.2±25.6pg/ml;辐照后24、48、72h,血清s CD30浓度随辐射剂量增大而降低。回归分析显示,血清s CD30浓度与辐照剂量具有明显的剂量-效应关系(R^2=0.9913),其回归方程为y=1.1489x^2-21.139x+121.44。结论小鼠血清s CD30表达基线稳定,其浓度变化与急性辐照剂量相关,可作为γ电离辐射剂量估算的生物指标。Objective To assess the feasibility of serum soluble CD30（s CD30） as a biomarker for radiation exposure. Methods Three hundred male C57BL/6 mice were prepared and randomly divided into 4 radiation groups（60 each） and control（n=60）. Mice in radiation groups were treated with 60 Co total-body irradiation in the dose of 1, 3, 5 or 7 Gy, and the dose rate of 60 Co γ ray at one-time whole-body irradiation was 0.78 Gy/min. 24, 48, or 72 h after radiation, the serum samples were obtained（20 mice in each group at each time point）. The serum s CD30 concentrations were analyzed with custom-made antibody array, which was purchased from Ray Biotech. To further analyze the relationships of serum s CD30 concentration and radiation dose, regression analysis was used. Results The baseline level of s CD30 in serum of non-exposed animals was 121.2±25.61pg/ml. Moreover, serum s CD30 concentration was inversely related to the radiation dose during 72 h after exposure. Regression analysis showed an obvious dose-effect relationship between serum s CD30 concentration and radiation dose（24h after radiation, R^2=0.8715; 48 h after radiation, R^2=0.9024; 72 h after radiation, R^2=0.9913）. Using these equations, radiation exposure can be easily estimated by the serum s CD30 value. Conclusions The baseline level of serum s CD30 remains steady without radiation. Further regression analysis uncovers a strong correlation between serum s CD30 concentration and radiation dose, and serum s CD30 may be used to estimate radiation exposure as a biomarker.

关 键 词：辐射 电离 辐射剂量 抗原 CD30 生物学标记 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]