基于响应面法分析温度和盐度对虎斑乌贼受精卵孵化的联合影响  

Combined effects of temperature and salinity on hatchability of fertilized eggs of Sepia pharaonis based on response surface methodology

作　　者：黄建盛[1] 陈刚[1] 张健东[1] 王忠良[1] 周晖[1] 汤保贵[1] 吴富华[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《生态学杂志》2016年第2期446-452,共7页Chinese Journal of Ecology

基　　金：广东海洋大学大学生创新创业训练计划项目(CXXL2014010);广东省教育厅育苗工程(自然科学)(2013LYM-0038);广东省海洋渔业科技推广专项项目(A2010-08D04);广东省海洋渔业科技推广专项项目(A2010004-016(c))资助

摘　　要：根据中心复合试验设计（CCD）原理,在单因素试验的基础上,采用响应曲面分析法,观察了温度（24~32℃）与盐度（25~35）对虎斑乌贼受精卵孵化率及平均孵化时间的联合影响。结果表明：温度和盐度的一次、二次及互作效应对孵化率与平均孵化时间均有显著影响（P〈0.05）,随着温度的升高,孵化率呈先上升后下降的趋势,平均孵化时间呈减少的趋势;随着盐度的上升,孵化率呈先上升后下降的趋势,平均孵化时间呈先下降后上升的变化;所考察的因子与孵化率、平均孵化时间之间二次多项模型的决定系数分别达到0.9791和0.9774;回归方程优化结果显示,温度为27.6℃和盐度31.1时,孵化率最大值为88.11%;温度26.2℃和盐度29.6时,平均孵化时间最小值为21.8 d,两模型的可信度均高达0.985。建议虎斑乌贼受精卵在温度26~28℃,盐度29~31条件下进行孵化,以提高生产效率。Using central composite design （CCD） and response surface methodology, the com- bined effects of temperature （ 24- 32 ℃） and salinity （ 25 - 35 ） on hatchability and average hatching time of fertilized eggs from Sepia pharaonic were evaluated on the basis of single-factor tests. The results showed that the linear and quadratic effects of temperature and salinity and their interactive effects significantly affected hatchability and average hatching time （P〈 0.05）. The hatchability increased first and then decreased as temperature or salinity increased. The average hatching time decreased as temperature increased, while it increased after an initial increase as salinity increased. The model equation of hatchability and average hatching time towards temperature and salinity were established, with the coefficients of determination （R2） being 0.9791 and 0.9774, respectively. The optimized results of regression equation based on a reliability of 0.985 showed that the hatchability was 88.11% when temperature was 27.6 ℃ and salinity was 31.1, and the average hatching time was 21.8 d when temperature was 26.2℃ and salinity was 29.6. Our results suggest that the hatching of fertilized eggs of S. pharaonis can be improved by rearing with an optimal combination of temperature and salinity with the range of 26-28℃ and 29-31,respectively .

关 键 词：虎斑乌贼 孵化率 平均孵化时间 中心复合设计 响应曲面 

分 类 号：S917.4[农业科学—水产科学]