自噬在体外醛固酮诱导的大鼠系膜细胞增殖中的作用  

Effect of autophagy in aldosterone-induced proliferation of rat mesangial cells in vitro

作　　者：施会敏 张爱青[1] 殷勤[1] 李善文[1] 甘卫华[1] 

机构地区：[1]南京医科大学第二附属医院儿肾科,江苏省210003

出　　处：《江苏医药》2016年第3期259-261,265,共4页Jiangsu Medical Journal

基　　金：南京市医学科技发展项目(YKK14180);南京医科大学科技发展基金重点项目(2014NJMUZD068)

摘　　要：目的探讨自噬在体外醛固酮诱导的大鼠系膜细胞增殖中的作用。方法体外培养大鼠系膜细胞,分为醛固酮组和对照组,分别应用倒置显微镜、透射电子显微镜及Western blot法观察醛固酮对系膜细胞形态、自噬水平及对自噬标志蛋白微管相关蛋白轻链3(LC3)和p62表达的影响。结果与对照组相比,醛固酮组系膜细胞形态未见明显变化,但细胞自噬体和自噬泡数量明显增加。醛固酮组LC3-Ⅱ和p62蛋白相对表达量高于对照组(1.81±0.07vs.1.00±0.04和2.75±0.03vs.1.00±0.04)(P<0.05)。结论醛固酮在诱导系膜细胞增殖的同时可引起自噬现象的激活,提示自噬可能是增殖状态下系膜细胞的一种自我保护机制。Objective To investigate the effect of autophagy in aldosterone-induced proliferation of rat mesangial cells in vitro. Methods Rat mesangial cells were cultured in vitro and divided into control group and aldosterone group. Effects of aldosterone on mesangial cells morphology and autophagy were determined by inverted microscope and transmission electron microscopy. The expressions of autophagy-related protein LC3 and p62 were determined by Western blot. Results Compared with control group, the morphology of mesangial cells was not obviously changed, but the number of autophagosome was increased in aldosterone group. The protein expressions of LC3-Ⅱ and p62 were significantly higher in aldosterone group than those in control group （1.81 ±0. 07 vs. 1.00±0. 04 and 2. 75±0. 03 vs. 1.00±0. 04） （P〈0. 05）. Conclusion Aldosterone can activate the autophagy during mesangial cells proliferation induced by aldosterone, which indicates that autophagy may be a self-protection mechanism of the cells under proliferation.

关 键 词：自噬 系膜细胞 醛固酮 细胞增殖 

分 类 号：R729[医药卫生—儿科]