液相色谱-串联高分辨质谱测定鸡肉中卡巴氧、喹乙醇的代谢物残留  

Determination of Metabolites of Carbadox and Olaquindox in Chicken by LC-HRMS

作　　者：陆桂萍[1] 曲斌[1] 耿士伟[1] 蒋天梅[1] 

机构地区：[1]江苏省畜产品质量检验测试中心,江苏南京210036

出　　处：《中国家禽》2016年第2期33-36,共4页China Poultry

摘　　要：本研究建立了鸡肉中卡巴氧、喹乙醇的代谢物残留的液相色谱-串联高分辨质谱测定方法。鸡肉样品经酸解后,固相萃取净化,液相色谱-串联四极杆静电场轨道阱高分辨质谱测定,稳定同位素内标定量。卡巴氧、喹乙醇的代谢物喹喔啉-2-羧酸、3-甲基-喹喔啉-2-羧酸在0.5μg/kg^10 g/kg范围内线性关系良好,最低定量限0.5μg/kg,检测限0.2μg/kg。高中低浓度精密度<20%(RSD%),平均回收率70%~120%。经实际样品的测定,本方法简便、实用,适宜于大批量样品的测定。An LC-HRMS method was established to determine the metabolites of carbadox and olaquindox in chicken. The samples were acidified,and cleaned-up by SPE. The analytes,quinoxaline-2-carboxylie acid （QCA）and methyl-3- quinoxaline-2-carboxylic acid （MQCA）, metabolites of carhadox and olaquindox, were determined by LC-quadrupole Orbitrap MS. Stable isotope was used as internal standard. QCA and MQCA were showed good linearity at the range between 0.5 μg/kg and 10 μg/kg. The limit of quantitation was 0.5 μg/kg. The intra- and inter-precision were lower 20% and the average recovery was between 70% and 120%. After practice of real samples,the method was simple and suitable to high throughput large scale samples.

关 键 词：卡巴氧 喹乙醇 液相色谱-串联高分辨质谱 残留 

分 类 号：S859[农业科学—临床兽医学] O657[农业科学—兽医学]