钛表面三维纳米多孔结构对细胞增殖、分化的影响  

Effects of Three Dimensional Nano Porous Titanium Film on Proliferation and Differentiation of Bone Mesenchymal Stem Cells in Vitro

作　　者：葛永梅[1] 宁成云[1] 徐彩霞[1] 谭帼馨[2] 刘绪建[1] 王玉强[1] 郑华德[1] 

机构地区：[1]华南理工大学特种功能材料教育部重点实验室,广东广州510641 [2]广东工业大学,广东广州510006

出　　处：《稀有金属材料与工程》2011年第S2期221-224,共4页Rare Metal Materials and Engineering

基　　金：国家自然科学基金项目(50872035)

摘　　要：采用阳极氧化法在纯钛表面构建三维纳米多孔膜层。利用SEM观察膜层表面形貌,XRD测定膜层成分。通过对人骨髓间质干细胞(hMSCs)在多孔膜层表面粘附、增殖和分化能力的检测,评价纳米多孔结构对骨髓间质干细胞粘附、增殖和分化的影响。结果表明,阳极氧化可以在钛表面制备出互相贯穿的三维纳米多孔网络膜层。膜层由钛酸钠构成。与纯钛表面相比,三维纳米多孔钛表面具有更优异的生物相容性,能够明显促进hMSCs的粘附、增殖和分化。A three dimensional nano-porous film on titanium has been prepared by anodic oxidation in NaHCO3 aqueous solution. SEM was used to characterize the film morphology and its component was demonstrated by XRD. The effects of three dimensional nano-porous film on the proliferation and differentiation of hMSCs were evaluated in vitro. The results show that a film composed of multiple layers of overlapped pores with three dimensional structure could be prepared. The main phase of the film was sodium titanate. In-vitro test indicates that three dimensional nano-porous film exhibits a better biocompatibility than titanium, which promotes the attachment, proliferation and differentiation of hMSCs.

关 键 词：钛 阳极氧化 纳米多孔 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]