复荣通脉胶囊对糖尿病模型大鼠坐骨神经白细胞介素-1及肿瘤坏死因子-α表达的影响  

Effects of Furong-Tongmai capsules on the expressions of interleukin-1 and tumor necrosis factor-α in the sciatic nerve in diabetic rats

作　　者：王立新[1] 王元松[1] 田风胜[1] 崔荣岗[1] 边云[1] 贾彩霞[1] 王庆凯[1] 

机构地区：[1]河北医科大学附属沧州中西医结合临床医学院内分泌科,沧州061001

出　　处：《国际中医中药杂志》2016年第1期53-56,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的观察复荣通脉胶囊对糖尿病模型大鼠坐骨神经IL-1、TNF-α表达的影响。方法50只大鼠按随机数字表法随机分为正常组、模型组及复荣通脉低、中、高剂量组，每组10只。采用腹腔注射STZ（60mg／kg）诱导糖尿病。诱导糖尿病后1周，复荣通脉低、中、高剂量组分别灌胃复荣通脉胶囊混悬液0．7、1．4、2．8g/kg，正常组和模型组灌胃等体积生理盐水，1d／次，共8周。采用免疫组化染色法检测坐骨神经IL-1和TNF-α表达水平。结果模型组IL-1[（1．43±0．17）％比（0．21±0．09）％；P〈0．05]和TNF-α[（1．98±0．12）％比（0．35±0．03）％；P〈0．05]表达均较正常对照组显著增高。复荣通脉胶囊中、高剂量组IL-1[（0．54±0．14）％、（0．51±0．13）％比（1．43±0．17）％；P〈0．05]、TNF-α[（0．57±0．17）％、（0．49±0．15）％比（1．98±0．12）％；P〈0．05]均较模型组显著降低，且显著低于复荣通脉低剂量组（IL-1：1．08％±0．18％，TNF-α：1．11％±0．09％；P〈0．05）。结论复荣通脉胶囊可降低糖尿病大鼠坐骨神经IL-1和TNF-α表达。Objective To investigate the effects of Furong-Tongmai capsules on the expressions of interleukin-1 （IL-1） and tumor necrosis factor-α （TNF-α） in the sciatic nerve in diabetic rats. Methods A total of 50 rats were randomly divided into the normal control group, model group, and low-, medium-, and high-dose of Furong-Tongmai groups, 10 rats in each group. Diabetes mellitus in rats were induced by intraperitoneal streptozotocin injection （60 mg/kg）. The rats in the low-, medium-, and high-dose of FurongoTongmai groups were intragastric administrated with Fumng-Tongmai suspension 0.7, 1.4 and 2.8 g/kg daily for 8 weeks, respectively. The rats in the normal control group and model group were intragastric administrated with equal-volume normal saline daily for 8 weeks. The expression levels of IL-1 and TNF-α in the sciatic nerve were detected with immunohistochemistry staining. Results The expression levels of IL-1 （1.43% ± 0.17% vs. 0.21% ± 0.09%; P〈0.05） and TNF-α （1.98% ± 0.12% vs. 0.35% ± 0.03%; P〈0.05） in the model group were significantly increased than those in the normal control group. The expression levels of IL-1 （0.54% ± 0.14%, 0.51% ± 0.13% vs. 1.43% ± 0.17%; all P〈0.05） and TNF-α （0.57% ± 0.17%, 0.49% ± 0.15% vs. 1.98% ± 0.12%; all P〈0.05） in the medium-, and high-dose of Furong-Tongmai groups were significantly decreased than those in the model group and low-dose of Furong-Tongmai group （1.08% ± 0.18% in IL-1, 1.11% ± 0.09% in TNF-α ; all P〈0.05）. Conclusion Furong-Tongmai capsules can reduce the expressions of IL-1 and TNF-α in sciatic nerve in diabetic rats.

关 键 词：糖尿病 实验性 糖尿病神经病变 中草药 白细胞介素-1 肿瘤坏死因子-Α 大鼠 复荣通脉胶囊 

分 类 号：R285.5[医药卫生—中药学]