Meta-ANOVA方法在FTO基因rs9939609多态性位点遗传模式界定中的应用  

Application of Meta-ANOVA approach in defining genetic model of rs9939609 in FTO gene polymorphism

作　　者：戚敏杰[1] 刘莉[2] 陈燕子[1] 詹芳芳[1] 平智广[1] 

机构地区：[1]郑州大学公共卫生学院卫生统计学教研室,郑州450001 [2]郑州大学基础医学院组织学与胚胎学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2016年第1期14-18,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目81001280;81202277;河南省高等学校重点科研资助项目16A330003

摘　　要：目的：探索FTO基因rs9939609位点的遗传模式,综合评价该位点多态性与肥胖的遗传关联性。方法：全面检索国内外数据库,搜集符合纳入标准的研究资料,并按照排除标准排除。运用meta-ANOVA方法对FTO基因rs9939609位点AA、AT和TT 3种基因型携带者的体重指数（BMI）的平均水平进行比较,得到设定的参数λ判断该位点的遗传模式。结果：共纳入文献48篇,按照年龄进行分层后,少年组9篇,成年组33篇,老年组6篇。3组的AA、AT和TT 3种基因型携带者BMI平均水平差异有统计学意义[少年组（P〈0.001）,λ=0.5,β=0.656,95%CI（0.489~0.823）;成年组（P〈0.001）,λ=0.5,β=0.670,95%CI（0.562~0.778）;老年组（P〈0.001）,λ=0.5,β=0.523,95%CI（0.363~0.682）]。3组的验证遗传模式均为加性遗传。结论：FTO基因rs9939609位点多态性与肥胖有关联性,A等位基因有使BMI增加的风险,该位点的遗传模式为加性遗传。Aim： To explore the genetic model of rs9939609 in FTO gene,as well as evaluate the association of gene polymorphism and obesity. Methods： All studies on this subject were searched in the electronic databases of China and abroad. The inclusion and exclusion criteria were used to select proper studies. The average level of body mass index（ BMI）was compared between groups of AA,AT and TT. Then a parameter λ was set to estimate the genetic model. Results： A total of 48 studies were included. They were stratified into 3 groups by age of subjects. Nine studies were in youth group,33 were in adult group,and 6 were in old group. The difference of BMI between AA,AT and TT genotype in three groups were statistically significant[in youth group（ P〈0. 001）,λ = 0. 5,β = 0. 656,95% CI（ 0. 489- 0. 823）; in the adult group（ P〈0. 001）,λ = 0. 5,β = 0. 670,95% CI（ 0. 562- 0. 778）; in old group（ P〈0. 001）,λ = 0. 5,β = 0. 523,95% CI（ 0. 363-0. 682） ]. The genetic model in three groups were all proved to be additive inheritance. Conclusion： The SNP rs9939609 on FTO gene is significantly associated with obesity. Allele A increases the risk of high BMI. The genetic model is confirmed to be additive.

关 键 词：meta-ANOVA 遗传模式 关联研究 基因多态性 FTO基因 

分 类 号：R181.2[医药卫生—流行病学]