解钾菌解钾效率检测方法的比较  

Comparing different methods of detecting potassium solubilizing efficiency with potassium solubilizing bacteria

作　　者：王珣珏 黄巧云[1] 蔡鹏[1] 陈雯莉[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2016年第1期81-85,共5页Journal of Huazhong Agricultural University

基　　金：国家重点基础研究发展计划("973"计划)项目(2015CB150504);教育部长江学者和创新团队发展计划项目(IRT1247);中央高校基本科研业务费专项(2662015PY016;2013PY111;2013PY136)

摘　　要：以中慢生根瘤菌S-15和类芽胞杆菌S-17作为供试菌株,采用细胞破碎、NH4OAc浸提、H2O2溶液消煮及不作任何前处理等4种方法,利用火焰光度计检测解钾菌发酵液中K+含量,并计算解钾菌在培养基中的解钾效率。结果表明,配制的3种钾系列标准溶液所绘制的钾标准曲线较为接近,R2分别高达0.994 4、0.9997、0.999 8。采用H2O2消煮后所测得的K+浓度最高,2株解钾菌的解钾效率分别达到101.1%、125.1%,与其他处理组之间有显著性差异。用H2O2溶液处理所得到的解钾率更能真实反映解钾菌的解钾作用。Using Mesorhizobiumsp.S-15 and Paenibacillus sp.S-17 as experimental strains.After cell disruption,NH4 OAc extraction,H2O2 solution digestion or without any pre-treatment,K+content in the fermentation broth of potassium solubilizing bacteria was determined with flame photometer.The efficiency of K solubilizing by potassium solubilizing bacteria in the culture medium was calculated.The results showed that the standard curves of the three kinds of K series of standard solutions were similar with the R2 values of 0.994 4,0.999 7and 0.999 8,respectively.After digested by H2O2 solution,concentration of K+was detected.The efficiency of two strains was 101.1%and 125.1%,significantly higher than that of other groups.The efficiency of potassium solubilizing detected after H2O2 digestion well reflected potassium solubilization of bacteria.

关 键 词：解钾菌 解钾效率 钾离子 生物钾肥 前处理方法 检测方法 

分 类 号：S154.3[农业科学—土壤学]