leptin基因在团头鲂成鱼各组织和早期发育中的表达分析  

Expression analysis of leptin genes in adults' tissues and during early development in Megalobrama amblycephala

作　　者：赵鸿昊[1,2] 李学华[1] 曾聪[1] 王卫民[1,2] 董在杰[3] 高泽霞[1,2] 

机构地区：[1]华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室/农业部淡水生物繁育重点实验室,武汉430070 [2]淡水水产健康养殖湖北省协同创新中心,武汉430070 [3]中国水产科学研究院淡水渔业研究中心,无锡214081

出　　处：《华中农业大学学报》2016年第1期92-98,共7页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31472271);国家大宗淡水鱼类产业技术体系建设项目(CARS-46-05);中央高校基本科研业务费专项(2014PY013);农业部淡水渔业与种质资源利用重点实验室开放课题(KF201308)

摘　　要：应用荧光定量PCR方法检测团头鲂leptin及其相关基因—瘦素受体(leptin receptor,leptin-R)和瘦素受体重叠转录产物类似物1基因(leptin receptor overlapping transcript like-1,leprotl-1)在胚胎发育早期及成鱼各组织的表达量。结果显示,3个基因在团头鲂成鱼所有检测组织中均有所表达,表明3个基因在团头鲂机体中具有多重生理功能。早期发育过程中的表达量结果显示leptin-R和leprotl-1基因在胚胎阶段的表达水平变化趋势一致,而leptin在破膜后5d前一直处于较低并呈波动性变化;出膜后12d时leptin-R和leprotl-1基因表达量均达到了峰值,而leptin基因从破膜后第5天开始表达量持续上升,在第15天到达峰值,表明3个基因可能涉及到胚胎发育早期阶段的能量调控。Pearson相关分析显示3个基因在团头鲂胚胎发育早期中的表达均为显著正相关关系(P<0.05)。In the present study,the expression levels of the leptin and its related genes including leptin receptor(leptin-R)and leptin receptor overlapping transcript like-1(leprotl-1)in Megalobrama amblycephala during embryonic and larval development as well as in various adults' tissues were studied using qRT-PCR.The results showed that the three genes were ubiquitously expressed in all the examined adults' tissues,which suggested their multiple physiology functions in M.amblycephala.During embryonic and larval developmental,the leptin-Rand leprotl-1genes had lower and fluctuant expression levels.Expression levels of the three genes had no differences before 5days post-hatching(dph),which suggested that these genes may be related to the regulation of energy during early developmental stages.Expression levels of both the leptin-Rand leprotl-1genes reached the peak at 12 dph,but the leptin expression increased from 5dph and reached the peak at 15 dph,which indicated that the three genes may participated in the M.amblycephalasex differentiation during larval developmental stages.The correlation coefficients among leptin,leptin-Rand leprotl-1mRNA levels indicated that these three genes were positively correlated during embryonic and larval developmental stages in M.amblycephala.

关 键 词：团头鲂 LEPTIN基因 leptin-R基因 leprotl-1基因 早期发育 组织 表达模式 

分 类 号：S917[农业科学—水产科学] Q959.468[生物学—动物学]