天津滨海新区泡桐丛枝病植原体16S rDNA基因序列分析  

Sequence analysis of 16S rDNA of phytoplasma causing paulownia witches'-broom in Binhai New District of Tianjin

作　　者：胡佳续[1] 唐芳[1] 张莹[1] 姜一[1] 赵璟源 刘鹏[1] 廖芳[1] 

机构地区：[1]天津出入境检验检疫局,天津300461

出　　处：《中国植保导刊》2016年第1期5-9,81,共5页China Plant Protection

基　　金："十二五"国家科技支撑计划课题(2012BAK11B02-2);天津出入境检验检疫局科研课题(TK071-2013)

摘　　要：利用分子生物学技术对天津滨海新区泡桐丛枝病病原进行分类鉴定。采用植原体16S rDNA通用引物R16mF2/R16mR1对患病植株总DNA进行PCR扩增,得到约1.4 kb特异性片段。克隆测序、Blast比对和iPhyClassifier分析结果表明,天津滨海新区泡桐丛枝植原体16S rDNA基因片段长1 432 bp,与国内泡桐丛枝植原体PY株系相似性最高,达99.86%,归属于16SrI组(aster yellows group,翠菊黄化组)D亚组。系统树构建与分析显示,泡桐丛枝病天津滨海株PaWB-TJBH与16SrI其他亚组亲缘关系较近,同在16SrI组进化枝上,与16Sr I-D组亲缘关系最近;16S rDNA序列RFLP电子酶切图谱表明,PaWB-TJBH属于16SrI-D组一个成员,与同源性比较和系统进化分析结果一致。Pathogenic agent of paulownia witches＇-broom（PaWB-TJBH） collected from Binhai New District of Tianjin was identified by molecular biology.Universal phytoplasma primer pairs R16mF2/R16mR1,were used to amplify 16 S rDNA by PCR,specific fragment about 1.4 Kb was yielded.Based on cloning,sequencing,blast searching,phylogenetic tree and iPhyClassifier analysis,the results showed that PaWB-TJBH consisted of 1432 bp in 16 S rDNA gene,which shared the highest similarity（99.86%） with strain of PaWB-PY,and belonged to aster yellows group（16SrI-D subgroup）.The above results identified classification status of PaWB-TJBH isolate,which provided a reference for epidemic regularity,prevention and control of phytoplasma-induced disease.

关 键 词：泡桐丛枝病 植原体 16S RDNA 序列分析 

分 类 号：S763.18[农业科学—森林保护学]