玉米CMS–C型雄性不育系C543恢复基因的初定位  

Primary mapping of restoring gene for CMS?C cytoplasmic male sterility line C543 in maize

作　　者：刘伟华[1] 罗红兵[1,2] 邱博[1] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]湖南农业大学南方粮油作物协同创新中心,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2016年第1期16-19,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金："十二.五"农村领域国家科技计划项目(2011BAD35B01);湖南省科技计划项目(2013TP4096);长沙市科技计划项目(K1406002–61)

摘　　要：以玉米恢复系614、B137分别与CMS–C型雄性不育系C543组配的F2代(614群体和B317群体)为试材,进行花粉育性鉴定和遗传分析。花粉育性镜检发现,C543花药内没有花粉,属于无花粉型雄性不育;遗传分析表明,C543的不育性状由1对隐性核基因控制,恢复系614和B137各有1对核恢复基因;SSR标记连锁分析结果将恢复基因定位在玉米8号染色体短臂上,位于标记umc1483附近,两者距离约16.9 c M。Two F2 generations, which from the hybridizations of C543×614 and C543×B137, were used as experiment materials to analyze the pollen fertility and inheritance. The results showed that C543 was pollen-free male sterility, which was controlled by a pair of recessive nuclear genes, and there was a restoring gene in restorer 614 and B137, respectively. With the analyzing of SSR, the restoring gene of B137 was mapped on short arm of chromosome 8 and 16.9 c M from the marker umc1483.

关 键 词：玉米 雄性不育系 质核互作 连锁分析 基因定位 

分 类 号：S513[农业科学—作物学] Q78[生物学—分子生物学]