基于线粒体DNAD–loop序列的黄郎鸡遗传多样性与品种起源研究  

Genetic diversity and breed origin of Huanglang chicken inferred from mitochondrial DNA D–loop sequence

作　　者：黄勋和[1] 翁茁先[2] 李威娜[1] 陈洁波[1] 钟福生[1] 唐寿桂 赵宇旗 

机构地区：[1]嘉应学院生命科学学院,广东梅州514015 [2]湖南农业大学动物科技学院,湖南长沙410128 [3]衡山县畜牧水产局,湖南衡阳421300 [4]衡山湘旗农牧有限责任公司,湖南衡阳421341

出　　处：《湖南农业大学学报（自然科学版）》2016年第1期75-80,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：广东省自然科学基金项目(2014A030307018);嘉应学院"创新强校工程"项目(CQX019);嘉应学院重点科技计划项目(2015KJM03);广东省公益研究与能力建设项目(2015A020208020);国家星火计划项目(2013GA780066)

摘　　要：采用PCR产物直接测序法对黄郎鸡50个个体的线粒体DNA D–loop序列进行分析,统计黄郎鸡周边省份地方鸡的进化支地理分布特征。结果表明,黄郎鸡具有较高的线粒体遗传变异水平,检测到26个变异位点,核苷酸多样性为0.012 54±0.000 94,核苷酸差异均数为6.569,单倍型多态性为0.886±0.035。在定义的23种单倍型中,13种单倍型为首次发现,单倍型落在A、B、C、E共4个进化支,B为优势单倍群。系统进化分析和进化支的地理分布特征表明,黄郎鸡起源于中国北方和西南地区,同时受邻省地方鸡基因交流的影响。Fifty Huanglang chickens were selected to directly sequencing using the PCR products. Geographic distribution of the clades of chickens from China was analysis. The results showed that Huanglang had high levels of genetic diversity, with nucleotide diversity, nucleotide differences and haplotype diversity were 0.012 54±0.000 94, 6.569 and 0.886±0.035, respectively. In the 26 variable sites, 23 haplotypes were defined, 13 of which was new. These haplotypes were belonging to clade A, B, C and E, respectively, and E was dominated haplogroup. Phylogenetic analysis and geographic distribution of the clades suggested that Huanglang originated from north and southwest China, and the gene flow with neighbor chickens was contributed significantly to this breed formation.

关 键 词：黄郎鸡 D–loop序列 遗传多样性 单倍型 地理分布 

分 类 号：S831.2[农业科学—畜牧学]