HB13对大鼠的一般生殖毒性研究  

General reproductive toxicity of HB13 on rats

作　　者：龚夏实[1] 张彤[1] 李燕[1] 惠晓琳 李继洪[1] 

机构地区：[1]湖北省医药工业研究院有限公司湖北省药物安全性评价中心,武汉430061

出　　处：《广州医药》2016年第1期20-23,共4页Guangzhou Medical Journal

摘　　要：目的观察HB13对SD大鼠的一般生殖毒性。方法 SD大鼠,雌雄各100只,分为低、中、高剂量组(20、40和80 mg/(kg·d))和对照组(0.5%羧甲基纤维素钠溶液),每组25只。雄鼠于交配前28 d给药,雌鼠于交配前14天给药,给药至妊娠第7天。雄鼠交配后处死,孕鼠于妊娠第14天颈椎脱臼处死,观察HB13对雌雄鼠一般情况、生育力和胚胎发育的影响。结果与对照组相比,HB13高、中剂量组雄鼠给药期体重减轻,睾丸系数及附睾系数增大,异常精子率升高;高剂量组还使雄鼠生育力降低。给予高、中剂量HB13的雌鼠妊娠后体重较对照组减轻,着床后丢失率增高,吸收胎数增加,活胎数减少,连胎子宫重降低;高剂量组HB13还使雌鼠生育率及着床数降低,着床前丢失率升高。结论 HB13低剂量对雌、雄性大鼠无一般生殖毒性;中剂量对孕鼠早期胚胎发育有明显干扰作用;高剂量对雌、雄大鼠生育力有显著降低作用并且对早期胚胎发育有明显干扰作用。Objective To study the general reproductive toxicity of HB13 in SD rats. Methods SD rats, 100 females and 100 males, were divided into low, medium and high dose groups 20, 40 and 80 mg/ （kg·d） and the control group （0. 5% CMC-Na） , The rats were given HB13 for 28 days in male rats and 14 days in female rats respectively before mating, and then mated. The HB13 treatment continued until the 7th day of pregnancy. When the female rats were confirmed pregnant, the male rats were executed and the female rats were executed on the 14th day of pregnancy. Then we can observe the effect of HB13 on fertility and embryonic development in rats. Results Compared with the control group, the body weight of medium, high dose group reduced significantly, the coefficient of testicular and epididymis, abnormal sperm rate increased significantly. High dose group of HB13 also made the fertility of male rats decline; The body weight, live births uterus weight （including the fetus） of pregnant rats in high and medium group decreased significantly. At the same time, the rate of lost oosperm and absorbed embryo increased. The high dose group of HB13 can also reduce the fertility of female rats. Conclusion The low dose group of HB13 didn＇t have general reproductive toxicity in rats, the medium dose group impaired early embryonic development, but the high dose group can significantly reduce the fertility of both male and female rats, and can impair the development of embryonic.

关 键 词：HB13 生殖毒性 大鼠 生育力 胚胎发育 

分 类 号：R114[医药卫生—卫生毒理学]