nm23-H1和p53基因在子宫内膜异位症患者异位组织中的表达及意义  

Expression and implication of nm23-H1 and p53 gene in endometriosis

作　　者：汪根莲 林爱琴[2] 段仁杰[2] 李铁臣[2] 孙青[1] 

机构地区：[1]皖南医学院第一附属医院弋矶山医院妇产科,安徽芜湖241001 [2]皖南医学院分子生物学研究室,安徽芜湖241002

出　　处：《皖南医学院学报》2016年第1期5-7,共3页Journal of Wannan Medical College

基　　金：安徽省高校省级自然科学研究重点项目(KJ2014A267)

摘　　要：目的:通过对子宫内膜异位症患者的异位内膜组织和正常对照组子宫内膜组织中nm23-H1基因和p53基因mRNA表达水平的研究,探讨nm23-H1基因和p53基因在子宫内膜异位症发生中的作用以及两基因表达的相关性。方法:收集30例子宫内膜异位症患者的异位内膜组织和30例正常对照组子宫内膜组织标本,用qRT-PCR方法检测nm23-H1基因和p53基因在两组中的表达,并分析两基因表达的相关性。结果:1相比于正常子宫内膜组织,nm23-H1基因和p53基因在异位内膜组织中的表达均下调,其表达差异具有统计学意义(t=2.575,P=0.015 vs t=2.708,P=0.011)。2nm23-H1基因和p53基因在子宫内膜异位症患者异位内膜组织中的表达有一定相关性(r=0.42,P=0.02)。结论:nm23-H1基因和p53基因mRNA表达水平的下降可能与子宫内膜异位症的发生、发展相关。nm23-H1基因可能通过p53途径导致子宫内膜异位症的发生,其确切机制仍有待进一步研究。Objective： To investigate the mRNA expression level of nm23-H1 and p53 gene in subjects with normal endometrium or endometriosis for clarifying the roles of the two genes in the pathogenesis and development of endometriosis. Methods： Endometrium samples were retrospectively obtained from healthy women（ n = 30） and patients with endometriosis（ n = 30）. Real-time quantitative PCR was performed to determine the mRNA expression level of nm23-H1 and p53 gene in the two groups,and the relationship between the two genes were analyzed. Results： By comparison with the normal endometrial tissues,patients with endometriosis had down-regulated mRNA expression levels of nm23-H1 and P53 gene（ t = 2. 575,P = 0. 015 vs t = 2. 708,P = 0.011） in which the two genes were correlated to a certain extent（ r = 0. 42,P = 0. 02）. Conclusion： Down-regulated levels of nm23-H1 gene and p53 gene may be associated with the occurrence and development of endometriosis,and nm23-H1 gene leading to endometriosis may be involved in the alteration of p53. However,the exact mechanism remains to be further clarified.

关 键 词：子宫内膜异位症 NM23-H1 P53 REAL-TIME q PCR 

分 类 号：R711.71[医药卫生—妇产科学]