用于激光捕获显微切割的组织冰冻切片制作方法探讨  

Preparation of frozen tissue sections for laser capture microdissection

作　　者：段仁杰[1,2] 林爱琴[2] 汪根莲 李铁臣[2] 孙青[1] 

机构地区：[1]皖南医学院第一附属医院弋矶山医院妇产科,安徽芜湖241001 [2]皖南医学院分子生物学研究室,安徽芜湖241001

出　　处：《皖南医学院学报》2016年第1期16-17,21,共3页Journal of Wannan Medical College

基　　金：安徽省高校省级自然科学研究重点项目(KJ2014A267)

摘　　要：目的:探讨经样本保存液处理的组织用于激光捕获显微切割技术冰冻切片的制作技术与方法。方法:以手术中切取的子宫内膜组织为标本。经样本保存液处理后,置于冰箱冷冻保存,经切片、染色,用于激光捕获显微切割。结果:在传统切片的基础上,改良后的切片更适用于激光捕获显微切割及相关实验。结论:改良的切片满足了激光捕获显微切割技术的要求和组织学观察的精确性。Objective： To investigate the technology and approaches to producing a frozen section for laser capture microdissection. Methods： Surgically resected endometrial tissue was obtained. Then the specimens were treated with preservation solution and frozen in an icebox,and subjected to section and staining for laser capture microdissection. Results： On conventional section preparation basis,the modified profile led to excellent quality of tissue sections for laser capture microdissection or use in related experiment. Conclusion： Our improved technology can result in quality sections to satisfy the laser capture microdissection and accurate histological observation.

关 键 词：激光捕获显微切割 冰冻切片 切片染色 

分 类 号：R318.51[医药卫生—生物医学工程]