玉米根际土球孢白僵菌(Beauveria bassiana)的分离与鉴定  

Isolation and Identification of Beauveria bassiana From Maize Rhizosphere Soil

作　　者：陈方新[1] 梅玉云 张强[1] 齐永霞[1] 丁婷[1] 

机构地区：[1]安徽农业大学植物保护学院,安徽合肥230036

出　　处：《核农学报》2016年第1期58-64,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金面上项目(11008382);安徽农业大学稳定和引进人才科研资助项目(2011);安徽农业大学青年基金资助项目(2011-31)

摘　　要：为明确虫生真菌在玉米根际的分布情况,通过选择性培养基对采自安徽省涡阳、萧县、蒙城3地的玉米根际土壤中的虫生真菌进行了分离,并结合形态特征及r DNA ITS序列分析对分离的菌株进行了鉴定。结果表明,从玉米根际土壤中共分离得到39个白僵菌菌株,经鉴定均为球孢白僵菌(Beauveria bassiana),且不同菌株间的菌落生长速度和分生孢子产生量存在显著差异。本研究对丰富玉米地下害虫及土传病害的生物防治资源具有重要的意义。To identify the distribution of entomogenous fungi in the maize rhizosphere,the entomogenous fungi which were collected from counties of Guoyang,Xiaoxian,and Mengcheng in the northern of Anhui Province were isolated from maize rhizosphere soil by selective medium. The isolates were classified and identified by morphological characters and molecular method（ r DNA ITS sequence analysis）. The results revealed that thirty-nine entomogenous fungi identified as Beauveria bassiana were isolated from maize rhizosphere soil,and there were significant differences in the colony growth rate and sporulation quantity of different strains. It has important significance in bio-controlling underground pests and soil-borne diseases in maize.

关 键 词：球孢白僵菌 分离 鉴定 分生孢子产生量 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]