利用MiSeq测序技术分析锦江牛瘤胃细菌多样性  

Analysis of Rumen Bacterial Diversity in Jinjiang Cattle Using MiSeq Sequencing Technology

作　　者：高雨飞 欧阳克蕙[1] 瞿明仁[1] 熊小文[1] 温庆琪[1] 许兰娇[1] 

机构地区：[1]江西农业大学江西省动物营养重点实验室,营养饲料开发工程研究中心,南昌330045

出　　处：《动物营养学报》2016年第1期244-248,共5页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31260561);国家现代农业产业技术体系(nycytx-38)

摘　　要：本研究旨在利用MiSeq高通量测序技术揭示锦江牛瘤胃细菌组成及多样性。选用3头装有永久性瘤胃瘘管的锦江牛成年公牛[体重（400±20）kg],采集瘤胃液样品后提取细菌DNA,对瘤胃细菌的16S rDNA序列V4-V5区进行MiSeq测序,分析物种的丰度、分布及α-多样性。结果表明：3个样本共获得56 763条高质量16S rDNA序列,946个分类操作单元（OTU）,锦江牛瘤胃细菌丰富度指数Chao指数、Ace指数分别为836、841,α-多样性指数Shannon指数、Simpson指数分别为4.96、0.021 5;经过物种注释,15个门、23个纲、26个科以及44个属在所有样品中被鉴定。在门水平,拟杆菌门（Bacteroidetes）（64.37%）、厚壁菌门（Firmicutes）（21.20%）和变形菌门（Proteobacteria）（7.59%）数量最多;在纲水平,主要包括拟杆菌纲（Bacteroidia）（52.88%）和梭菌纲（Clostridia）（17.03%）;在科水平,主要包括普雷沃氏菌科（Prevotellaceae）（36.26%）、瘤胃菌科（Ruminococcaceae）（9.33%）和毛螺菌科（Lachnospiraceae）（4.89%）;在属水平,优势细菌主要包括普雷沃氏菌属（Prevotella）（28.97%）、帕拉普氏菌属（Paraprevotella）（3.08%）、理研菌属（Rikenella）（2.28%）、梭菌Ⅳ（ClostridiumⅣ）（1.77%）、解琥珀酸菌属（Succiniclastium）（1.62%）和瘤胃球菌属（Ruminococcus）（1.53%）。结果证明,锦江牛瘤胃细菌多样性相对较低;锦江牛瘤胃中最优势菌门是拟杆菌门,其次是厚壁菌门;普雷沃氏菌属是锦江牛瘤胃中最优势细菌属。The present study was designed to reveal the rumen bacterial diversity in Jinjiang cattle using MiSeq high-flux sequencing technology. Ruminal liquid samples were obtained from three Jinjiang cattles（ bull）weighted（ 400 ± 20） kg with permanent rumen cannulas. Total DNA was extracted and the 16 S rDNA V4 to V5 of bacteria was sequenced by M i Seq sequencing technology. The abundance,distribution and α-diversity index of species were analyzed. The results showed that a total of 56 763 valid 16 S rDNA sequences and 946 operational taxonomic unit（ OTU） across 3 samples were obtained. The richness indexes,Chao and Ace indexes,of ruminal bacterial were 836 and 841,respectively; and alpha diversity indexes,Shannon and Simpson indexes were 4.96 and 0.021 5,respectively. Fifteen phyla,23 classes,26 families and 44 genera were identified via taxonomic summary. The dominant phyla among them were Bacteroidetes（ 64. 37%）,Firmicutes（ 21.20%） and Proteobacteria（ 7.59%）. The most abundant classes were Bacteroidia（ 52.88%）,Clostridia（ 17.03%）,and Prevotellaceae（ 36.26%）. Ruminococcaceae（ 9.33%） and Lachnospiraceae（ 4.89%） were the predominant family. At genus level,the high abundance of genera were Prevotella（ 28.97%）,Paraprevotella（ 3.08%）,Rikenella（ 2.28%）,Clostridium Ⅳ（ 1.77%）,Succiniclastium（ 1.62%） and Ruminococcus（ 1.53%）. In conclusion,the diversity of rumen bacterial community in Jinjiang cattle is low. Bacteroidetes is the predominant phylum and Firmicutes is the second most abundant phylum. Prevolla is the most abundant genus in the rumen bacterial community.

关 键 词：锦江牛 瘤胃细菌 多样性 MiSeq测序 

分 类 号：S823[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]