p38丝裂原活化蛋白激酶信号通路抑制剂在慢性间歇性缺氧大鼠软腭重构作用的研究  

Effect of p38MAPK inhibitor on the soft palate reconstruction of the rats with chronic intermittent hypoxia

作　　者：葛丽荞[1] 明婷[2] 闫静[3] 侯瑾[3] 巩楠[1] 赵琳[1] 王翡[1] 

机构地区：[1]西安医学院第一附属医院耳鼻咽喉头颈外科,陕西西安710077 [2]西安医学院第一附属医院麻醉科,陕西西安710077 [3]西安交通大学第二附属医院耳鼻咽喉头颈外科,陕西西安710004

出　　处：《中国耳鼻咽喉头颈外科》2016年第1期49-52,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：陕西省自然科学基础研究计划项目(2013JC2-22);陕西省教育厅专项科研计划项目(12JK0759)联合资助

摘　　要：目的探讨p38MAPK抑制剂SB203580对慢性间歇性缺氧大鼠软腭重构的保护作用。方法总数60只雌性SD大鼠,被随机分为3组:对照组、缺氧组和缺氧+SB203580干预组。缺氧组和缺氧+SB203580干预组大鼠在自动控制间歇性缺氧试验箱饲养,均饲养35 d后,取大鼠软腭组织HE染色,免疫组化方法测定p38MAPK的表达,Western blot检测软腭组织p38MAPK和p-p38MAPK的表达。结果缺氧组软腭组织厚度增加最明显,缺氧+SB203580干预组软腭组织厚度增加程度减轻;缺氧组软腭组织中p38MAPK表达增高最明显,缺氧+SB203580干预组p-p38MAPK减低明显。结论 p38抑制剂SB203580通过减轻软腭组织中p-p38的表达,从而减轻慢性间歇性缺氧大鼠软腭的损伤。OBJECTIVE To explore the role of P38 MAPK inhibitor on the soft palate reconstruction of the rats with chronic intermittent hypoxia.METHODS The animals were divided into normal control group,hypoxia control group and SB203580＋hypoxia group（every group 20 rats）.After 5 weeks,the expressions of p38 MAPK and p-p38 MAPK protein on the soft palate of the rats were detected with immunohistochemical techniques and western blot.RESULTS Compared with the normal control group,the soft palate tissue thickness of the hypoxia group were increased most obviously;Levels of p38 MAPK were increased in hypoxia control group;Compared with the hypoxia control group,the levels of p-p38 MAPK group were decreased in SB203580＋hypoxia group.CONCLUSION p38 MAPK may play important roles in the soft palate reconstruction of the rats with chronic intermittent hypoxia.

关 键 词：缺氧 腭 软 免疫组织化学 P38丝裂原活化蛋白激酶类 重构 

分 类 号：R766[医药卫生—耳鼻咽喉科]