扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用及机制研究  

Study of the Anti-tumor Effect and Mechanism of Strengthening Healthy Qi and Eliminating Pathogenic Factors on Mice Model with Lewis Lung Cancer

作　　者：齐元富[1] 张盈盈[2] 李慧杰[1] 郑祎[2] 刘寨东[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250014 [2]山东中医药大学,山东济南250355

出　　处：《山东中医杂志》2016年第1期61-63,共3页Shandong Journal of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划(编号:2009Z003)

摘　　要：目的:研究扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用并探讨其作用机制。方法:建立Lewis肺癌小鼠移植瘤模型,按实验分组干预,计算各组抑瘤率,免疫组化法检测BCL-2表达情况,RT-PCR法检测Caspase-3 m RNA、Survivin m RNA表达情况。结果:芪贞固本方、芩莲正积方均有不同程度的抑瘤作用,其抑瘤率分别为18.42%、29.24%,两组比较差异无统计学意义(P>0.05);二方均可促进Caspase-3 m RNA的表达,抑制BCL-2、Survivin m RNA的表达,与对照组比较有显著统计学意义(P<0.05),但均不及DDP组。结论:芪贞固本方和芩莲正积方确有抗肿瘤作用,芩莲正积方作用略强,其机制可能为促进Caspase-3m RNA的表达,抑制BCL-2、Survivin m RNA的表达。Objective：To study of the anti-tumor effect and mechanism of strengthening healthy Qi and eliminating pathogenic factors on mice model with Lewis lung cancer. Method：The Lewis lung cancer mouse model was established,the mic were divided into different groups and given intervention. The anti-tumor rate of each group was calculated,the expression of BCL-2 in tumor cells was measured with immunohistochemistry,the expressions of caspase-3 mRNA,surviving mRNA were detected with real-time PCR. Results：Qizhen guben formula and Qinlian zhengji formula all had certain anti-tumor effect,with the anti-rate 18.42% and 29.24% respectively,and there were no significant differences between two groups（P〉0.05）. When compared to the control group,they all could significantly promote the expression of caspase mRNA and down-regulate the expression of BCL-2 survivin mRNA,but the their effect was not better than cisplatin. Conclusion：Qizhen gubeu formula and Qinlian zhengji formula did possess anti-tumor effect and this effect of Qinlian Zhengji formual was stronger, Their mechanism of action may be related to promoting the expression of caspase mRNA and down-regulating the expression of BCL-2 and survivin mRNA.

关 键 词：扶正法 祛邪法 肺癌 机制 小鼠 

分 类 号：R285.6[医药卫生—中药学]