高效液相色谱法测定胃乐胶囊中硫酸阿托品含量  

Determination of Atropine Sulfate in Weile Capsules by HPLC

作　　者：李德潮[1] 

机构地区：[1]广西医科大学制药厂,广西南宁530021

出　　处：《中国药业》2016年第2期68-69,共2页China Pharmaceuticals

摘　　要：目的建立测定胃乐胶囊中硫酸阿托品含量的高效液相色谱（HPLC）法。方法色谱柱为Kromasil C_（18）柱（250 mm×4.6 mm,5μm）,流动相为0.05 mol/L磷酸二氢钠溶液（用磷酸调节pH至2.8）-乙腈（90∶10）,柱温为30℃,流速为1.0 mL/min,检测波长为210 nm。结果硫酸阿托品质量浓度在10.0~100.0μg/m L范围内与峰面积呈良好线性关系,平均回收率为98.88%,RSD为0.50%（n=6）。结论该方法准确、灵敏、重复性好,可作为胃乐胶囊的质量控制方法。Objective To establish an HPLC method for the determination of atropine sulfate in Weile Capsules.M ethods The chromatographic column was the Kromasil C_（18） column（250 mm×4.6 mm,5μm） at the temperature of 30 °C,with 0.05 mol/L sodium dihydrogen phosphate solution（adjusting pH to 2.8 with phosphoric acid） — acetonitrile（90-10） as the mobile phase and detected with UV 210 nm and the flow rate of 1.0 mL/min.Results The Atropine Sulfate in the range of 10.0- 100.0μg/mL showed good linear relatiomship with the peak area,the average recovery rate was 98.88%,RSD = 0.50%（n = 6）.Conclusion This method is accurate,sensitice and reproducible,which can be used for the determination of atropine sulfate in Weile Capsules.

关 键 词：高效液相色谱法 胃乐胶囊 硫酸阿托品 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]