24-乙酰泽泻醇A对氧化型低密度脂蛋白诱导巨噬细胞脂代谢因子ABCA1、CD36及炎症因子CD147、MMP-9的影响  

The Effect of Alisol A 24-acetate on Lipid Metabolic Factors ABCA1,CD36 and Inflammatory Factors CD147, MMP-9 in Oxidized Low Density Lipoproteininduced Macrophages

作　　者：施凤飞[1] 魏伟[2] 汪玉成[1] 苏清平[1] 薛偕华[2] 

机构地区：[1]福建中医药大学康复医学院,福建省福州市350003 [2]福建中医药大学附属康复医院神经康复科,福建省福州市350003

出　　处：《中国动脉硬化杂志》2016年第1期7-12,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金面上项目(81473744);福建省卫生厅中医药科研项目(WZSY201304);福建省卫生系统中青年骨干人才培养项目(2013-ZQN-ZD-28)

摘　　要：目的通过体外实验探讨24-乙酰泽泻醇A对氧化型低密度脂蛋白(ox-LDL)诱导大鼠腹腔巨噬细胞脂代谢因子ATP结合盒转运体A1(ABCA1)、B族清道夫受体(CD36)和炎症因子细胞外基质金属蛋白酶诱导因子(CD147)、基质金属蛋白酶9(MMP-9)蛋白表达的影响。方法分别采用50 mg/L ox-LDL和10 mg/L Dil-ox-LDL诱导巨噬细胞,10 mg/L 24-乙酰泽泻醇A进行干预。荧光显微镜观察细胞内Dil-ox-LDL蓄积情况;蛋白免疫印迹检测细胞ABCA1、CD36、CD147、MMP-9蛋白的表达。结果 10 mg/L Dil-ox-LDL诱导后大鼠腹腔巨噬细胞内有大量的Dil-ox-LDL蓄积,10 mg/L 24-乙酰泽泻醇A干预后,细胞内Dil-ox-LDL蓄积明显减轻。与对照组比较,50 mg/L ox-LDL诱导后大鼠腹腔巨噬细胞ABCA1、CD36和CD147、MMP-9蛋白表达明显增加,10 mg/L 24-乙酰泽泻醇A干预后,ABCA1蛋白表达进一步上升(P<0.01),CD36、CD147和MMP-9蛋白表达被明显抑制(P<0.05或P<0.01)。结论 24-乙酰泽泻醇A上调巨噬细胞的脂代谢因子ABCA1和抑制CD36的表达,减少胆固醇蓄积,同时抑制炎症因子CD147和MMP-9的分泌。Aim To evaluate the effect of Alisol A 24-acetate on the protein expression of lipid metabolism fac- tors ATP-binding cassette transporter A1 （ ABCA1 ）, class B scavenger receptor （CD56） and inflammatory factors extracel- lular matrix metalloproteinase inducer （ CD147 ）, matrix metalloproteinase-9 （MMP-9） in oxidized low density liprotein （ox-LDL）-stimulated rat peritoneal macrophages. Methods Rat peritoneal macrophages were respectively treated with 50 mg/L ox-LDL and 10 mg/L Dil-ox-LDL, and intervened with 10 mg/L Alisol A 24-acetate. Dil-ox-LDL accumulation in macrophages was observed with fluorescence microscope. The protein expression of ABCA1, CD147, CD36 and MMP-9 were detected by Western blot. Results After induced with 10 mg/L Dil-ox-LDL, a large number of Dil-ox- LDL accumulation was observed in peritoneal macrophages of rats. Intracellular Dil-ox-LDL accumulation was significantly reduced after 10 mg/L Alisol A 24-acetate intervention. Compared with the control group, the protein expressions of ABCA1, CD36 and CD147, MMP-9 were significantly increased in peritoneal macrophages after induced with 50 ox-LDL mg/L. After 10 mg/L Alisol A 24-acetate intervention, the protein expression of ABCA1 was increased further （P 〈 0. 01 ）, and protein expressions of CD36, CD147 and MMP-9 were significantly inhibited （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusions Alisol A 24-acetate can increase the expression of hpid metabolic factor ABCA1, inhibit the expression of CD36, and reduce cholesterol accumulation in macmphages. Also it can inhibit the secretion of inflammatory factors CD147 and MMP-9.

关 键 词：24-乙酰泽泻醇A 巨噬细胞 ABCA1 CD36 CD147 MMP-9 

分 类 号：R96[医药卫生—药理学]