抑制干扰素诱导蛋白p204表达促进大鼠血管外膜成纤维细胞生长与迁移  

Inhibition of p204 Expression Promotes Rat Vascular Adventitia Fibroblasts Growth and Migration

作　　者：宋方[1] 田茂波[1] 黄晶[1] 龙向淑[1] 吴强[1] 

机构地区：[1]贵州省人民医院心内科,贵州省贵阳市550002

出　　处：《中国动脉硬化杂志》2016年第1期18-22,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助项目(81260030)

摘　　要：目的观察干扰素诱导蛋白p204表达抑制后对大鼠血管外膜成纤维细胞(VAF)凋亡、增殖与迁移的影响及其部分机制。方法应用Ifi204小干扰RNA(si RNA)转染VAF使Ifi204基因沉默(Ifi204-si RNA组),以非特异性si RNA转染作为其转染阴性对照组(Con-si RNA组)和未经处理的VAF作为未干预阴性对照组(Neg组)。用MTT法检测VAF细胞增殖,流式细胞仪检测细胞凋亡,细胞划痕法和Transwell法测定细胞迁移情况,应用实时荧光q RT-PCR和Western blot分别检测p204、p53及p21的m RNA和蛋白表达。结果与Con-si RNA组和(或)Neg组相比,Ifi204-si RNA组的p204、p53及p21的m RNA和蛋白表达减少(P<0.05),细胞凋亡率下降(P<0.05),细胞增殖及迁移速度提高(P<0.05)。结论抑制p204表达可减少大鼠VAF细胞凋亡,促进其增殖与迁移,其机制可能部分与抑制p53及p21表达有关。Aim To observe the effect of interferon-inducible protein 200 （p200） on the apoptosis, proliferation and migration of vascular adventitial fibroblast cells （VAF） in rat. Methods The specific small interference RNA （siRNA） of p204 gene （Ifi204） were transfected into VAF in vitro instantaneously. VAF were divided into three groups, Ifi200 siRNA transfection group （Iti204-siRNA）, control siRNA transfection group （Con-siRNA）, untreated VAF as a nega- tive intervention control group （Neg）. Then the cell vitality to reflect cell proliferation was analyzed by MTF method, cell apoptosis was analyzed by flow cytometry, cell migration by scratch assay and transwell chamber. The mRNA and protein levels of p200, p53 and p21 were measured by real-time qRT-PCR and Western blot, respectively. Results After transfection of the Ifi204 siRNA, the mRNA and protein expression levels of p200, p53 and p21 were decreased in the VAF, and the cell apoptosis was inhibited, the cell proliferation and migration were promoted. Conclusion Inhibition of p200 expression can promote VAF cell growth and migration, which may be related to the inhibition of p53 and p21 expression.

关 键 词：干扰素诱导蛋白p204 血管外膜成纤维细胞 细胞凋亡 细胞增殖 细胞迁移 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学]