男性生殖道感染患者脂多糖对成熟精子直接损伤作用与机制研究  

Effect and mechanism of lipopolysaccharide of male patients with reproductive tract infections on direct damage of mature sperm

作　　者：刘萍[1,2] 向代军[2] 陈运霞[1,2] 薛丹丹[2] 张帆[2] 孙崇云[2,3] 王策[2,3] 梁臻龙[2,3] 刘晓婷[2] 王成彬[2] 

机构地区：[1]河北北方学院研究生部,河北张家口075000 [2]解放军总医院临床检验科,北京100853 [3]温州医科大学检验医学院生命科学院,浙江温州325035

出　　处：《中华医院感染学杂志》2016年第4期883-885,910,共4页Chinese Journal of Nosocomiology

基　　金：国家重大科学仪器设备开发专项基金资助项目(2012YQ18011708)

摘　　要：目的研究男性生殖道感染患者脂多糖(LPS)作用成熟精子后精子活力、活性氧以及凋亡指标的变化,探讨LPS对成熟精子的损伤情况,从而研究生殖道感染后影响男性生育能力的相关机制。方法通过上游法处理正常精液标本,调整浓度为5×106/ml的高活力精子重悬液,设置对照组和试验组,37℃孵育一定时间后检测精子活力、精子内活性氧、精子凋亡和线粒体膜电位等指标的变化情况。结果试验组精子内活性氧的平均荧光强度为559±42.7,线粒体膜电位的红色平均荧光强度/绿色平均荧光强度的比值为11.1±2.61,与对照组相比,精子内活性氧的升高和线粒体膜电位的降低差异均有统计学意义(P<0.05);但是并未增加精子的凋亡率和降低精子的活力。结论 LPS与高活力精子重悬液孵育作用于精子表面可能升高精子内活性氧,导致精子线粒体损伤,引起线粒体膜电位的降低,可能是男性生殖道感染LPS直接作用于精子参与影响男性不育的机制。OBJECTIVE To study the changes of sperm motility,reactive oxygen,and sperm apoptosis after the lipopolysaccharide（LPS）of male patients with reproductive tract infections acts on the mature sperm and explore the impact of LPS on damage of the mature sperm so as to observe the related mechanism of impact of reproductive tract infections on the male fertility.METHODS The normal semen specimens were treated by using swim-up method,the concentration was adjusted as 5×106/ml of high-vitality sperm resuspension,and the control group and the experimental group were set.The sperm motility,sperm reactive oxygen,sperm apoptosis,and mitochondrial membrane potential were detected after the high-vitality sperm resuspension was incubated at 37℃ for a certain time.RESULTS The mean fluorescence intensity of sperm reactive oxygen of the experimental group was 559±42.7,the ratio of red and green fluorescence intensity of mitochondrial membrane potential of the experimental group was 11.1±2.61,and there was significant difference in the increase of sperm reactive oxygen or decrease of mitochondrial membrane potential between the control group and the experimental group（P〈0.05）.However,they did not result in the increase of rate of sperm apoptosis or reduction of sperm vitality.CONCLUSIONThe LPS and high-vitality sperm resuspension act on the surfaces of sperm,which may lead to the increase of the sperm reactive oxygen and the damage of sperm mitochondria as well as the reduction of mitochondrial membrane potential.The LPS of the male patients with reproductive tract infections may play a direct role in the mechanism of the sperm′s participating the male infertility.

关 键 词：脂多糖 男性不育 精子 活力 凋亡 线粒体膜电位 

分 类 号：R691.3[医药卫生—泌尿科学]