白族药青阳参HPLC指纹图谱研究  

HPLC Fingerprint of the Traditional Bai Medicine Cynanchi otophylli Radix

作　　者：苏小军 杨怀镜 曾懿 闻焜 郑健[2] 过立农[2] 昝珂[2] 

机构地区：[1]云南省大理州食品药品检验所,大理671000 [2]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2015年第12期1299-1304,共6页Chinese Pharmaceutical Affairs

基　　金：中国食品药品检定研究院中青年发展研究基金(编号2013WA11)

摘　　要：目的：建立青阳参药材HPLC指纹图谱分析方法,研究青阳参药材的质量。方法：采用RP-HPLC色谱法,以Inertsil ODS-SP（4.6 mm×150 mm,5μm）为色谱柱,0.1%甲酸水溶液-乙腈为流动相梯度洗脱,柱温35℃,流速1.0 m L·min-1,检测波长采用波长切换,0~35 min为285 nm,35~80 min为进样量10μL;数据使用中药色谱指纹图谱相似度评价系统研究版（2012A）进行处理。结果：在选定的色谱条件下确定14个峰构成青阳参药材的特征共有峰,各批次药材均具有上述特征,但特征峰的相对含量分布差异导致色谱概貌存在一定差异。结论：该方法简便,精密度高、重现性好,可为青阳参药材的质量评价提供依据。Objective： To establish the HPLC fingerprint analysis method for Cynanchi otophylli Radix and to research the quality of Cynanchi otophylli Radix. Methods： The RP-HPLC method was applied in chromatographic separation, using an Inertsil ODS-SP column（4.6 mm×150 mm, 5 μm）, with 0.1% formic acid solution-acetonitrile at a flow rate of 1.0 m L·min-1 as the mobile phase, and the column temperature was 35 ℃. Multiple wavelengths were used to detect the signals, 0-35 min at 285 nm, 35-80 min at 270 nm, and the injection volume was 10 μL. The data were analyzed by ＂chromatographic fingerprint similarity evaluation＂ software（2012A）. Results： A total of 14 peaks were identified as the characteristic fingerprints of Cynanchi otophylli Radix. All samples tested contained the same 14 peaks, but the relative content distribution of the characteristic peaks showed great difference among the samples, which resulted in the differences in their chromatograms. Conclusion： This method is simple, accurate, highly reproducible, and can be used for the quality control of Cynanchi otophylli Radix.

关 键 词：白族药 青阳参 指纹图谱 HPLC 

分 类 号：R284[医药卫生—中药学]