HPLC法测定民族药材青阳参中对羟基苯乙酮的含量  

Determination of 4-Hydroxyacetophenone in Radix Cynanchi otophylli by HPLC

作　　者：昝珂[1] 郑健[1] 过立农[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2015年第12期1310-1314,共5页Chinese Pharmaceutical Affairs

基　　金：中国食品药品检定研究院中青年发展研究基金(编号2013NA7)

摘　　要：目的：建立反相高效液相法测定民族药材青阳参中对羟基苯乙酮的含量。方法：选用YMC-Pack ODS-A C18色谱柱（250 mm×4.6 mm,5μm）,流动相：甲醇-水（35∶65）,检测波长275 nm,流速：1.0 ml·min-1,柱温：30℃。结果：对羟基苯乙酮在0.506~20.24μg·m L-1范围内线性关系良好,精密度、稳定性、重复性、加样回收率试验RSD均小于2%,平均加样回收率为97.38%。结论：该方法准确、简便、灵敏度高,可作为青阳参药材中对羟基苯乙酮的含量测定方法。Objective： To establish an RP-HPLC method to determine the content of 4-hydroxyacetophenone in Radix Cynanchi otophylli. Methods： The separation was performed on a YMC-Pack ODS-A C18 column with methanol-water（35∶65） as mobile phase, the flow rate was 1.0 m L·min-1. The detection UV wavelength was at 275 nm, and the column temperature was 30 ℃. Results： The calibration curve of 4-hydroxyacetophenone was linear in the range of 0.506-20.24 μg·m L-1, the RSD of precision, solution stability, repeatability, recovery was less than 2%, and the average recovery was 97.38%. Conclusion： The method is simple, rapid and accurate, which can be used for the quality control of Radix Cynanchi otophylli.

关 键 词：青阳参 HPLC 对羟基苯乙酮 含量测定 

分 类 号：R927[医药卫生—药学] R931