苏淮猪群体抗腹泻基因MUC13和FUT1多态性分析及其抗腹泻选育方案研究  

Polymorphism analysis of anti-diarrhea genes MUC13 and FUT1 in Suhuai pigs and its selective breeding on anti-diarrhea traits

作　　者：方宇瑜 吴艳[1,3] 高硕[1,2] 李强[1,3] 李会智[1,2] 付玲玲[1,2] 任二都 李平华[1,2] 黄瑞华[1,2] 

机构地区：[1]南京农业大学养猪研究所,江苏南京210095 [2]南京农业大学淮安研究院,江苏淮安223005 [3]淮安市淮阴种猪场,江苏淮安223322

出　　处：《畜牧与兽医》2015年第12期12-17,共6页Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业科技自主创新项目CX(15)1006;江苏省农业三新工程项目SXGC[2014]138和SXGC[2015]319;淮安市科技支撑计划(农业)项目(SN13093)

摘　　要：腹泻是现代猪场集约化规模养殖过程中常见的疾病,给养殖场带来巨大的经济损失。产肠毒素大肠杆菌F4ac以及F18是导致仔猪腹泻的重要病原微生物,而MUC13和FUT1分别是控制仔猪大肠杆菌F4ac以及F18易感或抗性的主效基因。本试验对淮安市淮阴种猪场的苏淮猪核心群做了MUC13和FUT1基因的多态性检测。结果表明:在整个苏淮猪核心群中MUC13的G等位基因频率较高,其中公、母猪群体分别达到0.625和0.568,有利于苏淮猪抗腹泻性能的选育提高;然而FUT1的A等位基因频率较低,其中公、母猪群体分别为0.250和0.190。根据苏淮猪MUC13和FUT1基因多态性结果,制定了以MUC13基因的选择为主,FUT1基因选择为辅的抗腹泻群体的分子选育方案,以期通过结合常规育种手段来提高苏淮仔猪对腹泻的抗性。Diarrhea is a common disease in modem pig farm which causes significant economic losses. Enterotoxigenic Escherichia coli F4ac and FI8 are important pathogens that induce diarrhea of piglets. The major genes which control piglets＇ susceptibility to diarrhea are MUC13 and FUT1 genes. In this study, genotyping of MUC13 and FUT1 genes was scored in the core group of Suhuai pigs on Huaiyin pig breeding farm. The results showed that the frequency of G allele in MUC13 gene was high that the boars reached 0. 625 and the sows 0. 568. However, the frequency of A allele ＂in FUT1 gene was low and only 0. 250 in boars and 0. 190 in sows. According to the polymorphism of these two genes, a molecular breeding plan combination with both molecular and conventional breeding method was formulated to enhance the resistance of Suhuai piglets against diarrhea.

关 键 词：苏淮猪 腹泻 抗性基因 MUC13 FUT1 

分 类 号：S859.1[农业科学—临床兽医学]