过敏性休克小鼠血清及BALF中IL-4、IL-6和IgE水平检测及其临床意义  

IL-6,IL-4 and IgE levels in serum and BALF of anaphylactic shock mice

作　　者：陈洁[1] 谢华[2,3] 曹建平[2] 

机构地区：[1]大连医科大学研究生院,116000 [2]沈阳军区总医院呼吸与变态反应疾病诊治中心 [3]大连医科大学

出　　处：《浙江医学》2015年第23期1920-1922,1933,共4页Zhejiang Medical Journal

基　　金：辽宁省自然科学基金项目(201202244)

摘　　要：目的通过研究过敏性休克小鼠模型致敏组和对照组中IL-4、IL-6和IgE的不同表达,进一步探讨过敏性休克的免疫学发病机制,为人类过敏性休克的治疗提供新的思路。方法雄性昆明种小鼠60只随机分成致敏组40只和对照组20只。致敏组小鼠于第1天腹腔注射致敏原卯蛋白(OVA)0.25ml,1周后加强注射一次,饲养3周后尾静脉注射OVA 0.5ml诱发过敏性休克;对照组以同等量的PBS缓冲液代替。以酶联免疫吸附(ELISA)法检测致敏组和对照组小鼠血清及肺泡灌洗液(BAI-F)中IL-4、IL-6和IgE的水平。结果成功建立过敏性休克小鼠模型。致敏组血清IL-4、IL-6和IgE的水平分别是对照组的3.59、2.49、2.83倍。致敏组BAL-F中IL-4、IL-6和IgE的水平分别是对照组的2.75、1.82、1.58倍。致敏组血清及BALF中IL-4、IL-6和IgE水平两两呈正相关(均P<0.01);对照组血清及BALF中IL-4、IL-6和IgE水平均无关(均P>0.05)。结论血清及BALF中IL-4、IL-6水平升高提示细胞因子可能参与过敏性休克发病过程。IL-4、IL-6与IgE之间的相关性研究提示,IL-6可能通过上调IL-4的表达在过敏性休克的发病过程中发挥作用。Objective To investigate IL-6, IL-4 and IgE levels in serum and bronchioalveolar lavage fluid （BALF） of mice with anaphylactic shock. Methods Sixty male KM mice were randomly divided into sensitized （n=40） and control groups （n=20）. The mice in sensitized group were immunized by intraperitoneal injection of allergen at first day and then repeated after a week; allergic shock was induced by tail vein injection after three weeks later. The mice in control group were injected with equal volume of PBS buffer instead. The IL-6, IL-4 and IgE levels in serum and BALF were measured by ELISA. Results The mice model of anaphylactic shock was successfully established. Serum and BALF IL-6, IL-4 and IgE levels in sensitized group were increased by 3.59, 2.49, 2.83 times and by 2.75, 1.82, 1.58times, respectively. Serum and BALF levels of IL-6, IL-4 were positively correlated with those of IgE; and the IL-6 levels were also positively correlated with those of IL-4. In control group, there was no correlation of IL-6, IL-4 and IgE in serum and BALF with each other. Conclusion Increased serum and BALF levels of IL-6, IL-4 in sensitized mice indicate that the two cytokines are likely to participate in the pathogenesis of anaphylactic shock in mice.

关 键 词：过敏性休克 IL-4 IL-6 免疫球蛋白E 

分 类 号：R593.1[医药卫生—内科学]