不同产地板蓝根中(R,S)-告依春及核苷类成分含量的比较  

Comparative study on(R,S)-goitrin and nucleosides in Isatidis Radix derived from different regions

作　　者：池絮影 傅咏梅[2] 张蜀[1] 邓红[1] 崔曰新 宁晓辉[1] 胡雪艳[1] 朱秀城 

机构地区：[1]广东药学院药物研究所/广东省药物新剂型重点实验室,广东广州510006 [2]国药集团广东环球制药有限公司,广东佛山528200

出　　处：《广东药学院学报》2015年第6期745-748,共4页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的建立超高效液相色谱（UPLC）法同时测定板蓝根药材中尿苷、鸟苷、（R,S）-告依春、腺苷质量分数的方法。方法采用反相超高效液相色谱法,色谱柱为phenomenex Kinetex C_（18）柱（4.6 mm×100mm,2.6μm）,流动相为甲醇（A）-水（B）,梯度洗脱;流速为0.6 m L/min;检测波长为254 nm;柱温为30℃;进样量为2μL。结果尿苷、鸟苷、（R,S）-告依春和腺苷分别在2.058~102.9μg/m L（r=0.999 8）、2.070~103.5μg/m L（r=0.999 8）、2.043~102.2μg/m L（r=0.999 8）和2.506~125.3μg/m L（r=0.999 8）质量浓度范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为99.9%、99.7%、99.4%、100.6%,RSD值分别为0.42%、1.3%、0.97%、0.88%（n=6）。结论本方法简便、快捷、重复性好,可用于板蓝根药材的质量控制。不同产地或不同生长环境下同种板蓝根中（R,S）-告依春和核苷质量分数均存在明显差异。Objective To establish a method for the determination of uridine,guanosine,（ R,S）-goitrin and adenosine in Isatidis Radix by ultra-performance liquid chromatography（ UPLC）. Methods Phenomen Kinetex C_（18）（ 4.6 mm×100 mm,2.6 μm） column was used with mobile phase consisted of methanol（ A）-water（ B） by gradient elution at a flow rate of 0.6 m L/min and the detection wavelength was 254 nm. The column temperature was 30 ℃,and the injection volume was 2 μL. Results The linear range of uridine,guanosine,（ R,S）-goitrin and adenosine were 2. 058- 102. 9 μg/m L（ r = 0. 999 8）,2. 070- 103. 5 μg/m L（ r= 0.999 8）,2.043-102.2 μg/m L（ r = 0.999 8） and 2.506-125.3 μg/m L（ r = 0.999 8）,respectively.Their average recoveries（ n = 6） were 99.9%,99.7%,99.4% and 100.6% with RSD 0.42%,1.3%,0.97%and 0. 88%,respectively. Conclusion The method was simple,exclusive and accurate with excellent repeatability,which could be used for quality control of Isatidis Radix. The contents of epigoitrin and nucleoside varied markedly in samples from different regions.

关 键 词：板蓝根 尿苷 鸟苷 (R S)-告依春 腺苷 UPLC法 含量测定 

分 类 号：R284.1[医药卫生—中药学]