拟南芥染色质重塑因子编码基因INO80新的可变剪切转录本  

A Novel Alternative Transcript of Chromatin Remodeling Factor-Encoding Gene INO80 in Arabidopsis thaliana

作　　者：安增选 朱炎[1,2] 

机构地区：[1]复旦大学生命科学学院植物科学研究所,上海200438 [2]复旦大学遗传工程国家重点实验室,上海200438

出　　处：《复旦学报（自然科学版）》2015年第6期742-751,共10页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金(31271374)

摘　　要：可变剪切是基因转录本调控的重要方式之一,在植物中大量存在.研究表明可变剪切存在于植物不同发育阶段以及受应答环境变化的调控.INO80是一类保守的染色质重塑因子,在表观遗传学调控中发挥着重要的作用.我们在对拟南芥INO80展开功能分析时,意外发现了一个新的可变剪切转录本,At3G57300.3.通过定量PCR发现其表达具有一定的器官特异性,在不同条件处理时其转录水平也会发生变化.At3G57300.3编码了含有918个氨基酸的截短蛋白,在体外能够与H2A.Z-H2B结合,但缺乏完整的ATPase结构域,因而可能发挥着与全长蛋白不同的功能.Alternative splicing （AS） is an important pathway for transcript regulation and widely detected in plants. Numerous studies have indicated that AS exists in the various development stages and is regulated by environmental changes, INOS0 is a conversed chromatin remodeling factor playing key roles in epigenetic regulation. In this study, we found a novel alternative transcript of INCEO in Arabidopsis thaliana, At3G57300. 3. Quantitative PCR revealed that the relative transcription level of At3G57300. 3 displays organ specificity and responsiveness to different environmental conditions. At3G57300.3 encodes a truncated protein consists of 918 amino acid lacking of intact ATPase domain. The truncated protein can bind H2A. Z-H2B via INO80-Z domain in vitro. Therefore, it may exert different functions from fuU-length protein in vivo.

关 键 词：可变剪切 染色质重塑因子 INO80 H2A.Z 

分 类 号：Q5[生物学—生物化学]