蛇伤药酒的薄层鉴别及其盐酸小檗碱含量测定  

TLC Identification and Determination of Berberine Hydrochloride by HPLC in Sheshang Medicinal Liquor

作　　者：昌水平[1] 张健民[1] 王琦[2] 梁志云[1] 

机构地区：[1]江门市新会区中医院,广东江门529100 [2]江门市药品检验所,广东江门529000

出　　处：《江西中医药大学学报》2015年第6期57-59,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：新会区科技局项目(新科[2014]523号);江门市科技局项目(江科[2014]77号)

摘　　要：目的：研究蛇伤药酒的质量控制方法。方法：采用薄层色谱法（TLC）对制剂中大黄进行定性鉴别;采用高效液相色谱法（HPLC）测定盐酸小檗碱的含量,Diamonsil C_（18）（2）色谱柱（200mm×4.6mm,5μm）,流动相为乙腈-0.05mol/L磷酸二氢钾溶液（32：68）,检测波长为345nm,流速为1.0m L/min,柱温为25℃。结果：定性鉴别薄层色谱斑点特征明显;高效液相色谱法测定盐酸小檗碱含量,在0.04~0.42μg范围内与峰面积值呈良好的线性关系,回归方程：Y=0.6156X-0.0776（r=0.9999）,平均加样回收率为99.71%,RSD=1.44%（n=6）。结论：采用TLC法对大黄定性鉴别,HPLC定量测定盐酸小檗碱含量,两方法简便准确,重复性良好,可有效控制该制剂质量。Objective：To study the method of quality control for Sheshang medicinal liquor. Methods： The thin layer chromatography （TLC） was used to the qualitative identification of rheum officinale; the quantitative analysis of berberine hydrochloride was carried out by HPLC with a Diamonsil C,s （2） chromatograhpic column（200mm×4.6mm,5um） ,the mobile phase was acetonitrile - 0.05mol/L potassium dihydrogen phosphate solution （32：68） with the flow rate of 1.0 mL/min and the detection wavelength of 345nm, the tem- perature of column was 25 ℃. Results ： The characteristic of TLC spots was obvious in the qualitative identification. HPLC determind the content. The berberine hydrochloride in 0.04 - 0. 42ug extent show good linear ralation, and the regression equation was Y = 0. 6156X - 0.0776, （ r = 0.9999） with an average recovery rate of 99.71% （ RSD = 1.44%, n = 6）. Conclusion： The methods of using TLC and HPLC are simple and accurate, and can be used for the quality control of Sheshang medicinal liquor.

关 键 词：蛇伤药酒 高效液相色谱法 薄层鉴别 盐酸小檗碱 

分 类 号：R286.0[医药卫生—中药学]