BCG免疫对小鼠血清特异性抗体与脾脏免疫相关细胞因子表达的影响  

Effects of BCG Vaccination on Specific Antibodies in Serum and Expression of Immunity-related Cytokines in Spleen from Mice

作　　者：武月章 安潇潇[1] 张文慧[1] 张林波[1] 

机构地区：[1]吉林农业大学生命科学学院,长春130118

出　　处：《吉林农业大学学报》2015年第6期726-730,共5页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20130101105JC;20140204018YY)

摘　　要：为深入了解卡介苗诱导机体产生免疫效应的分子机制,对卡介苗(Bacillus calmettee-guerin,BCG)免疫后小鼠血清特异性抗体与脾脏免疫相关细胞因子表达变化及相关性进行了研究。利用BCG免疫C57BL/6小鼠,采集不同时间点的外周血和脾脏样本。应用间接ELISA方法检测血清中特异性抗体效价,实时荧光定量PCR方法检测脾脏中免疫相关细胞因子表达水平。结果表明:在BCG接种的后期,血清中抗体效价呈极显著的上升趋势(P<0.01);脾脏中Th1型细胞因子白细胞介素-2(Interleukin-2,IL-2)、γ干扰素(Interferon-γ,IFN-γ)的表达水平与对照组相比呈显著性上升(P<0.05),但Th2型细胞因子白细胞介素-4(Interleukin-4,IL-4)的表达水平则明显下降(P<0.05)。这说明BCG免疫后,机体会同时产生细胞免疫应答和体液免疫应答,但免疫后期Th1型细胞因子与体液免疫效应呈正相关,某些Th2型细胞因子则与体液免疫效应呈负相关性。In order to understand immune mechanism induced by Bacillus Calmettee-Guerin（ BCG）in vivo,experiments were carried out to study effects of BCG vaccination on specific antibodies in serum and expression of immunity-related cytokines in spleen from mice. Peripheral blood and spleen from C57 BL / 6 mice vaccinated with BCG were collected at different time points. Then specific antibodies in serum were detected by indirect ELISA,and expression of immunity-related cytokines in spleen were detected by real-time quantitative PCR. The results showed that value of specific antibodies was in a significant increase trend in the late stage of vaccination（ P 0. 01）. In addition,expression of Th1 type cytokine interleukin-2（ IL-2）,interferon-γ（ IFN-γ） was increased,and difference was significant（ P 0. 05）. However,expression of Th2 type cytokine interleukin-4（ IL-4） was reduced（ P〈0. 05）. These data suggest that organism can not only generate cellular immune responses but also humoral immunity responses in the late stage of BCG vaccination. During this stage,Th1 type cytokines are positively correlated with humoral immunity responses,but some Th2type cytokine is negatively correlated with it. These results may have theoretical significance to the study of BCG vaccine immune mechanism and the development of tuberculosis vaccines.

关 键 词：卡介苗免疫 特异性抗体 TH1型细胞因子 TH2型细胞因子 相关性 

分 类 号：S855.12[农业科学—临床兽医学]