苋菜AmaDOPA5-GT基因的启动子克隆、亚细胞定位及表达分析  

Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor

作　　者：郑学立[1,2] 刘生财[1] 谢礼洋[1] 程春振[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]福州市蔬菜科学研究所,福建福州350111

出　　处：《福建农业学报》2015年第11期1064-1070,共7页Fujian Journal of Agricultural Sciences

基　　金：高等学校博士学科点专项新教师类科研基金项目(20123515120009);福建省自然科学基金项目(2013j05045);福建省教育厅a类项目(ja12098)

摘　　要：对获得的苋菜环多巴5-糖基转移酶基因(AmaDOPA5-GT)进行生物信息学和蛋白亚细胞定位分析,并利用染色体步移技术克隆该基因的启动子。亚细胞定位结果表明,AmaDOPA5-GT定位于细胞膜。启动子分析结果表明AmaDOPA5-GT启动子中植物光响应元件多达8个,说明光对该基因的表达影响较大。色素含量测定及实时定量PCR结果显示:暗处理抑制苋菜苷合成和AmaDOPA5-GT表达;黄光和绿光抑制苋菜苷合成但对AmaDOPA5-GT表达影响不大;红光显著抑制苋菜苷合成但却显著诱导AmaDOPA5-GT表达;蓝光显著促进苋菜苷合成和AmaDOPA5-GT表达。本研究可为揭示AmaDOPA5-GT基因在苋菜苷的作用奠定基础并为富含甜菜红色素苋菜苷的苋菜育种提供线索。Bioinformatic characteristics and protein subcellular localization of AmaDOPA5-GT gene in Amaranth tricolor were studied.The chromosome walking technique was applied to clone the gene promoter.The protein was found to be located in the cytomembrane.Eight light-responsive elements were identified in the promoter,suggesting that light might be associated with the regulation of the gene expression.The amaranthin content and quantitative real time PCR showed that darkness suppressed both betalain biosynthesis and AmaDOPA5-GT gene expression;red light also suppressed betalain biosynthesis,but significantly enhanced the gene expression;yellow and green light showed no effect on the gene expression,but suppressed the betalain biosynthesis;and,blue light induced both betalain biosynthesis and the gene expression.The results obtained provided a basis for further exploring the function of AmaDOPA5-GT in betalain biosynthesis,as well as a guideline for breeding betalain-rich amarnath.

关 键 词：苋菜 DOPA5-GT 启动子 光质 甜菜红色素合成 

分 类 号：S636.4[农业科学—蔬菜学]