双层左旋聚乳酸静电纺织纳米纤维支架与人牙周膜细胞的生物相容性  

Biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold with human periodontal ligament cells

作　　者：孙文娟[1] 江浩顺[1] 黄南楠[1] 唐倩[1] 杨雨虹[1] 

机构地区：[1]中山大学附属第三医院口腔科,广东省广州市510630

出　　处：《中国组织工程研究》2015年第52期8438-8443,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:静电纺织制备的纳米纤维支架形态结构与天然细胞外基质相似,为细胞的生长、增殖提供了良好的微环境,可增强细胞的黏附、迁移、增殖及分化功能。目的:观察双层左旋聚乳酸静电纺织纳米纤维支架与人牙周膜细胞的生物相容性。方法:采用静电纺织技术制备双层左旋聚乳酸静电纺织纳米纤维支架。MTT法评估不同浓度(100%、75%、50%、25%)双层左旋聚乳酸静电纺织纳米纤维支架浸提液对人牙周膜细胞的毒性;将双层左旋聚乳酸静电纺织纳米纤维支架与人牙周膜细胞共培养,以MTT法检测细胞的早期黏附能力,扫描电镜观察细胞在支架上的生长情况。结果与结论:不同浓度双层左旋聚乳酸静电纺织纳米纤维支架浸提液对人牙周膜细胞无毒性。共培养2,6,24 h,人牙周膜细胞在双层左旋聚乳酸静电纺织纳米纤维支架上的黏附能力较差。复合培养7 d,人牙周膜细胞在支架疏松面黏附良好,并保持原有的形态,伸展良好,伸出突起相互连接;在支架致密面呈复层生长,胞体呈梭形、多角形,连接成片。表明双层左旋聚乳酸静电纺织纳米支架与人牙周膜细胞具有良好的生物相容性。BACKGROUND： The morphological structure of nanofiber scaffold which fabricated by electrospinning technique is similar to the natural extracellular matrix, which provides a good microenvironment for cell growth and proliferation, and can also enhance cell adhesion, migration, proliferation and differentiation. OBJECTIVE： To observe the biocompatibility of double-layer poly（L-lactic acid） electrospun nanofiber scaffold and human periodontal ligament cells. METHODS： Electrospinning technique was used to prepare double layers poly（L-lactic acid） electrospun nanofiber scaffold. The toxicity of different concentrations of（100%, 75%, 50%, 25%） double-layer poly（L-lactic acid） electrospun nanofiber scaffold extracts to human periodontal ligament cells was evaluated by MTT assay. The double-layer poly（L-lactic acid） electrospun nanofiber scaffold was co-cultured with human periodontal ligament cells. The cell adhesive capacity in early stage was determined by MTT assay. The growth of cells on the scaffold was observed by scanning electron microscopy. RESULTS AND CONCLUSION： Different concentrations of double-layer poly（L-lactic acid） electrospun nanofiber scaffold extracts did not create any toxicity to human periodontal ligament cells. After co-culture for 2, 6, 24 hours,human periodontal ligament cells were poorly adherent onto the double-layer poly（L-lactic acid） electrospun nanofiber scaffold. After 7 days of co-culture, human periodontal ligament cells adhered well on the loose surface of scaffold, maintained the original shape, stretched well, and interconnected processes were observed; on the dense surface of the scaffold, multi-layer cells were observed. The cells showed fusiform or polygonal appearance and were connected together. These results demonstrate that the double-layer poly（L-lactic acid） electrospun nanofiber scaffold has good biocompatibility with human periodontal ligament cells.

关 键 词：牙周膜 成纤维细胞 组织工程 生物材料 材料相容性 牙周组织工程 左旋聚乳酸 支架材料 屏障膜 静电纺织 人牙周膜细胞 

分 类 号：R318[医药卫生—生物医学工程]