ICU内泛耐药鲍曼不动杆菌耐消毒剂基因研究及消毒对策  

Detection of disinfectant-resistant gene in pan-drug-resistant acinetobacter baumanii in ICU and disinfection measures

作　　者：骆园园[1] 王厚照[1] 张玲[1] 

机构地区：[1]解放军第一七四医院(厦门大学附属成功医院)检验科,福建厦门361003

出　　处：《临床军医杂志》2015年第5期493-494,498,共3页Clinical Journal of Medical Officers

基　　金：全军医学科技青年培育项目(13QNP047);南京军区医学科技创新项目(2013ZD27);厦门市科技创新项目(3502Z20134024)

摘　　要：目的探讨ICU内泛耐药鲍曼不动杆菌(PDR-Ab)携带耐消毒剂基因的情况。方法采用聚合酶链反应(PCR)对PDR-Ab进行耐消毒剂基因的检测与分析。结果 75株PDR-Ab中73株携带耐消毒剂基因qac E△1-sul I,检出率为97.3%(73/75);12株携带qac A/B,检出率为16.0%(12/75);8株携带qac G,检出率为10.7%(8/75);未检出qac C或qac J基因阳性的菌株。结论 ICU内PDR-Ab耐消毒剂基因检出率高,主要以qac E△1-sul I为主,提示PDR-Ab可能对消毒剂存在抗性。Objective To investigate the detection of disinfectant-resistant gene in pan-drug-resistant acinetobacter baumanii（ PDRAb） in ICU. Methods PCR was used for detecting disinfectant-resistant gene in PDR-Ab. Results Of all these strains,97. 3%（ 73 /75） was found carrying qac E△1-sul I gene; 16%（ 12 /75） was found carrying qac A/B gene; 10. 7%（ 8 /75） was found carrying qac G gene. Neither qac C nor qac J gene was detected in any strains. Conclusion The positively detected rate of qac E△1-sul I gene in PDR-Ab in ICU is high. It is suggested that PDR-Ab may have disinfectant resistance.

关 键 词：泛耐药 鲍曼不动杆菌 耐消毒剂基因 消毒对策 重症监护室 

分 类 号：R378[医药卫生—病原生物学]