同种异体骨髓间充质干细胞静脉移植治疗脑梗死大鼠的疗效和机制研究  

Curative effect and mechanism of aliogeneic bone marrow mesenchymal stem cells therapy by intravenous transplantation for rats with cerebral infarction

作　　者：黄敏[1] 顾建娟[2] 赵春松[3] 赵仁超 关云谦[3] 李晓波[1] 

机构地区：[1]江苏省苏北人民医院神经内科,225001 [2]江苏省苏北人民医院妇产科,225001 [3]首都医科大学宣武医院细胞生物室教育部神经变性病重点实验室

出　　处：《神经疾病与精神卫生》2015年第6期559-564,F0003,共7页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金项目（81371377）;北京市科委健康培育项目（Z111107067311033）

摘　　要：目的探讨同种异体骨髓间充质干细胞（BM—MSCs）静脉移植治疗脑梗死大鼠的疗效及作用机制。方法90只SPF级SD雄性大鼠随机分为假手术组、对照组和MSC移植组，每组30只。采用大脑中动脉远端阻塞法（dMCA0）制备大鼠脑梗死模型，造模后1h经尾静脉移植MSC，移植后24h、48h行行为学评价，缺血后48h灌注取脑后测定梗死体积，用免疫组织化学法计数梗死周边皮层小胶质细胞数，并用ELISA法检测梗死灶周边皮层IGF-1,bFGF蛋白水平。结果移植后MSC移植组的脑梗死体积较对照组小，且行为学测试得分较对照组改善（P〈0．05）；梗死灶周边皮层小胶质细胞激活与对照组的差异无统计学意义（P〉0．05）；梗死周边皮层IGF-1,bFGF蛋白较对照组升高（P〈0．05）。结论BM—MSCs静脉移植可改善脑梗死大鼠的行为学表现，其机制可能与增加脑内营养性细胞因子有关。Objective To explore the curative effect and mechanism of allogeneic bone marrow mesenehymal stem cells （BM--MSCs） therapy by intravenous transplantation for rats with cerebral in- farction. Methods A total of 90 SD male rats ranked SPF were randomly divided into sham operation group （30 cases） ,control group （30 cases） and MSC group （30 cases）. Cerebral infarction models were made by distal middle cerebral artery occlusion （dMCAO）. One hour after that,the MSC was transplan- ted via the tail vein. The behavior was evaluated 24 h and 48 h after transplantation. We determined the infarction volume, counted microglia in the cortex in the periphery of the infarct by immunohistochemical method after taking out brain after 48 h of ischemia and measured the IGF- 1 in the same area by ELISA. Results The infarct volume was reduced and the score of behavior was higher in the MSC group than these in the control group （P〈0.05）. There was no significant difference in the activation of micro- glia in the cortex in the periphery of the infarct comparing with the control group （P^0.05）. IGF-- 1 and bFGF protein in the cortex in the periphery of the infarct was higher than that in the control group （P〈0.05）. Conclusions BM--MSCs vein transplantation can improve the behavior of rats with cerebral infarction, and its mechanism may be related to the increase of nutrient cell factors in the brain.

关 键 词：间充质干细胞 移植 脑梗死 小胶质细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]