急进高原后大鼠组织高原适应性基因含量的差异性观察  

Expression of plateau adaptation gene of rat tissues after plain acute exposure to high altitude

作　　者：谢华[1] 郝颖[1,2] 尹强[1] 李文斌[1] 鹿辉[1] 贾正平[1,2] 王荣[1] 

机构地区：[1]兰州军区兰州总医院,全军高原损伤防治重点实验室,甘肃兰州730050 [2]兰州大学生命科学学院,甘肃兰州730000

出　　处：《浙江大学学报（医学版）》2015年第5期571-577,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家科技重大专项(2008ZXJ09014-010);国家自然科学基金(81401552);全军医学科研“十二五”重点项目(BWS12J012)

摘　　要：目的：检测平原大鼠和急进不同海拔高原大鼠血液及组织器官中高原适应性基因（EPAS1、EGLN1和PPARα基因）含量及HIF-20t、PHD2和PPAROt蛋白表达量，初步探讨高原适应性基因及相应表达蛋白在急进高原大鼠体内心、肝、脑、肺、肾组织分布的差异。方法：实验大鼠分为平原组、急进高原3400m组、急进高原4300m组；急进高原两组到达高原第1、3、5天分别取大鼠血液及组织器官，检测血常规，采用实时定量PCR和ELISA方法检测高原适应性基因及相应蛋白表达量，并对其进行比较分析。结果：急进高原4300m组大鼠红细胞、血红蛋白、血细胞比容高于平原组（均P〈0．05）。与平原组比较，急进高原两组血液和心、肝、肾组织中EPAS1基因含量明显增加（均P〈0．05）；心、肝、脑、肾组织中EGLN1基因含量增加（均P〈0．05），心、肝、肾组织中PPARα基因含量增加（均P〈0．05）。与平原组比较，HIF-20t蛋白表达量在急进高原两组各组织器官中均增加，且高海拔处肝、脑、肾组织中增加明显；心、肝、肾组织中PHD2和PPARα蛋白表达量均增加（均P〈0．05）。结论：高原适应性基因的含量和相应蛋白表达量在不同海拔和不同组织中存在一定的差异。提示EPAS1、EGLN1和PPARα基因可作为抗高原缺氧药物靶向标志物。Objective： To detect the expression of the plateau adaptablity gene （EPAS1, EGLN1 and PPARα） and proteins （HIF-2, PHD2 and PPARα） in rats blood, heart, liver, lung and kidney tissue after the rats exposed to high altitude. Methods： The Wistar rats were randomly divided into plain group （Shanghai, 55 m） , acute exposure to high altitude 3400 m group, acute exposure to high altitude 4300 m group. Blood and organs of rats were collected in 1, 3, 5 days after arrival. Real time PCR and ELISA were used to compare the expression of plateau adaptablity gene and related protein between plain group and high altitude exposure groups. Results： The count of red blood cells, hemoglobin and HCT in high altitude 4300 m were higher than those in plain group. Compared with plain group, the expression of EPAS1 gene in blood, heart, liver and kidney tissue of rats at high altitude increased obviously （ all P 〈 0.05 ） ; the expression of EGLN1 in the heart, liver, brain and kidney increased, and PPARα gene in the heart, liver and kidney increased （ all P 〈 0.05 ）. Compared with plain group, the expression of HIF-2 protein increased significantly at high altitudes in the liver, brain and kidney tissues. PHD2 and PPARα increased in the heart, liver and kidney. Conclusion： Plateau adaptive genes （EPAS1, EGLN1 and PPARv） and protein （HIF-2, PHD2 and PPARα ） differed in different altitude and different organizations. They might be used as target markers of plateau hypoxia.

关 键 词：高原病/血液 高海拔 基因 大鼠 聚合酶链反应 

分 类 号：R339.5[医药卫生—人体生理学]