Surface molecularly imprinted polymers for solid-phase extraction of （-）-epigallocatechin gallate from toothpaste  

作　　者：Yunling Gao Ying Hu Kejian Yao 

机构地区：[1]State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China

出　　处：《Frontiers of Chemical Science and Engineering》2015年第4期467-478,共12页化学科学与工程前沿（英文版）

基　　金：Acknowledgements This work was supported by Zhejiang Provincial Natural Science Foundation of China （Grant No. LY 12B07010） and National Natural Science Foundation of China （Grant No.20807037）.

摘　　要：Surface molecularly imprinted polymers （SMIPs） have been synthesized to selectively determine （-）-epigallocatechin gallate in aqueous media. SMIPs were prepared using a surface grafting copolymerization method on a functionalized silica gel modified with β-cyclodextrin and vinyl groups. The morphology and composition of the SMIPs were investigated by scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analysis. In addition, the molecular binding capacity, recognition properties and selectivity of the SMIPs were evaluated. The imprinted polymers were found to have a highly specific recognition and binding capacity for aqueous media which is （-）-epigallocatechin gallate in the result of the hydrophobic properties of the β-cyclodextrin and the hydrogen-bonding interactions of methacrylic acid. The SMIPs were successfully employed as solid-phase extraction adsor- bents prior to the HPLC determination of （-）-epigallocatechin gallate in toothpaste. The HPLC analysis had a linear dynamic range of 0.5-50.0 μg·mL^-1 with a correlation coefficient of 0.9998 and the recoveries ranged from 89.4% to 97.0% with relative standard deviations less than 4.8%. The limit of detection and limit of quantification were 0.17 and 0.33 μg·mL^-1, respectively. The method provides a promising approach for the preparation of selective materials for the purification and determination of complex samples.Surface molecularly imprinted polymers （SMIPs） have been synthesized to selectively determine （-）-epigallocatechin gallate in aqueous media. SMIPs were prepared using a surface grafting copolymerization method on a functionalized silica gel modified with β-cyclodextrin and vinyl groups. The morphology and composition of the SMIPs were investigated by scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analysis. In addition, the molecular binding capacity, recognition properties and selectivity of the SMIPs were evaluated. The imprinted polymers were found to have a highly specific recognition and binding capacity for aqueous media which is （-）-epigallocatechin gallate in the result of the hydrophobic properties of the β-cyclodextrin and the hydrogen-bonding interactions of methacrylic acid. The SMIPs were successfully employed as solid-phase extraction adsor- bents prior to the HPLC determination of （-）-epigallocatechin gallate in toothpaste. The HPLC analysis had a linear dynamic range of 0.5-50.0 μg·mL^-1 with a correlation coefficient of 0.9998 and the recoveries ranged from 89.4% to 97.0% with relative standard deviations less than 4.8%. The limit of detection and limit of quantification were 0.17 and 0.33 μg·mL^-1, respectively. The method provides a promising approach for the preparation of selective materials for the purification and determination of complex samples.

关 键 词：Β-CYCLODEXTRIN （-）-epigallocatechin gallate surface molecular imprinting solid-phase extraction 

分 类 号：O641.3[理学—物理化学] TS272.4[理学—化学]