经血源子宫内膜干细胞复合3D打印PLGA支架体外培养的相容性研究  

Cellular Compatibility of Menstrual Blood-derived Mesenchymal Stem Cells in Three-dimensional Printing PLGA Scaffolds

作　　者：许世兵[1,2] 单乐天[1,2] 金红婷[1,2] 王萍儿[1,2] 童培建[1,3,2] 肖鲁伟[1,2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江省骨伤研究所 [3]浙江中医药大学附属第一医院

出　　处：《浙江中医药大学学报》2015年第12期854-858,共5页Journal of Zhejiang Chinese Medical University

基　　金：浙江省重点科技创新团队计划资助项目(2011R50022);浙江省重点实验室计划资助项目(2013E10024);浙江省重大科技专项重点社会发展项目(2014C03035);浙江省重点科技创新团队自主项目(2011R50022-02);中国博士后科学基金面上项目(2014M561793)~~

摘　　要：[目的]通过经血源子宫内膜干细胞(menstrual blood-derived mesenchymal stem cells,Men SCs)与3D打印PLGA支架材料的复合培养,探讨构建组织工程化骨软骨的可行性。[方法]预处理3D打印PLGA支架,取第5代Men SCs,倒置显微镜下观察细胞生物学特性,按1.0×106/m L的密度接种到PLGA支架材料上复合培养,通过倒置显微镜、Mico-CT、扫描电镜和组织病理学进行观察,并借此判断Men SCs与3D打印PLGA支架材料复合体外培养的融合性。[结果]Men SCs生长良好,细胞平铺生长,呈梭形或纺锤形,胞质薄,核圆居中,具有成纤维细胞形态。细胞传至第5代,为长梭形细胞单层,呈漩涡状排列。PLGA支架的微观呈现相互连通的多孔结构,结构疏松,孔隙大且互相交通,孔壁较薄。Men SCs在PLGA支架材料上生长状态良好,细胞在支架表面和内部生长,支架孔径内有细胞基质样组织连接,表明Men SCs与3D打印PLGA支架材料复合体外培养的融合性良好。[结论]经血源Men SCs是骨组织工程适宜的种子细胞,与3D打印的PLGA支架材料复合可体外构建组织工程骨。[Objective] To investigate the feasibility of using three-dimensional（3D） printing PLGA loaded with menstrual blood-derived mesenchymal stem cells（Men SCs） as scaffolds for bone cartilage tissue engineering. [Methods] Three-dimensional printing PLGA was preprocessed. The five generation Men SCs were utilized. use invert microscope to observe cells biological property. According to 1.0 ×10^6/m L cells seeded onto PLGA scaffold composite culture, invert microscope, Mico-CT, scanning electron microscope and histopathology were observed.[Results] MSCs grew well, cells were plated to grow,fusiform or spindle row, thin cytoplasmic and nuclear circle centered, with fibroblast morphology. Cells to the fifth generation had long spindle cell monolayer and swirling arrangement. PLGA scaffolds exhibited microscopic interconnected porous structure, loose structure, large pores and mutual traffic,thinner hole wall. Men SCs on PLGA scaffold grew well, mainly in the scaffolds surface and inside growth, the scaffolds aperture had cell matrix organization connection.[Conclusion] Men SCs are ideal seeding cells for bone cartilage tissue engineering. And the PLGA scaffolds by three-dimensional printing with Men SCs may be in vitro construction as bone cartilage formation.

关 键 词：子宫内膜干细胞 PLGA支架 经血 3D打印 骨组织工程 种子细胞 材料 

分 类 号：R331[医药卫生—人体生理学]