A型核纤层蛋白前体与RanBPM相互作用的验证  

Verification of interaction between prelamin and RanBPM

作　　者：高春海[1] 胡晓峰[1] 

机构地区：[1]山东省临沂市人民医院检验科,山东临沂276003

出　　处：《医学检验与临床》2015年第6期1-4,17,共5页Medical Laboratory Science and Clinics

基　　金：临沂市科学技术局基金项目（201413022）

摘　　要：目的：本实验室前期工作通过酵母双杂交技术初步证明RanBPM是一个与A型核纤层蛋白前体相互作用的蛋白，本研究进一步通过细胞外蛋白沉降实验（GST pull-down analysis）验证其细胞外的相互作用。方法：构建原核表达载体pET-32a-RanBPM，分别进行RanBPM和prelamin A的原核表达，通过GST pull-down实验证明两者在体外的相互作用。结果：成功构建原核表达载体pET-32a-RanBPM，通过GST pull-down实验验证了RanBPM和prelamin A在细胞外有相互作用。结论：RanBPM和prelaminA在细胞外有相互作用。Objective ： Several candidates, including vascular RanBPM, from human skeletal muscle cDNA library were found in our previous study by yeast two-hybrid screening. The purpose of this study was to identify the interaction between RanBPM and prelamin A by GST pull-down analysis. Methods ： Recombinant vector pET32a-RanBPM was constructed successfully and RanBPM was expressed in prokaryotic cells. Using the expressed RanBPM and prelamin A, GST puU-down assays was carried out to identify their interaction in vitro.Results ： The plasmid of pET-32a-RanBPM was constructed successfully and the interaction in vitro between prelamin A and NanBPM was confirmed by GST pull-down assays. Conclusions ： Our data demonstrated that RanBPM interact with prelamin A both in vitro and in vivo.

关 键 词：A型核纤层蛋白前体 RANBPM 细胞外蛋白沉降实验 

分 类 号：R541.4[医药卫生—心血管疾病]