临床疑难血型抗体病例分析探讨——附报告1例  

Analysis on clinical cases of difficult blood group antibody

作　　者：毕仕颖 苏品璨[2] 董伟群[1] 李毅[1] 吴穗[1] 

机构地区：[1]昆明医科大学第一附属医院输血科,云南昆明650032 [2]云南昆明血液中心

出　　处：《中国输血杂志》2015年第12期1518-1520,共3页Chinese Journal of Blood Transfusion

基　　金：云南省教育厅科学研究基金项目(2012Y020)

摘　　要：目的通过对疑难血型患者抗-E+抗-Jkb的鉴定,阐明抗体鉴定及交叉配血试验对输血的重要性。方法采用盐水法、聚凝胺法、抗球及卡式法进行血型血清学检测。结果血清学试验表明,患者的血清与谱细胞在盐水介质中没有发生凝聚,在抗球蛋白(AHG)介质中,与谱细胞中E抗原和JKb抗原阳性细胞发生凝集,与Jkb抗原阴性细胞未发生凝集,初步判定为抗-E+抗-Jkb。结论经过血型鉴定该患者为抗-E+抗-Jkb引起交叉配血不合,因此在临床工作中,应重视ABO血型系统以外的抗体的存在,采用多种方法学检测,以防漏检,可进一步提高输血安全性。Objective To identification anti E ＋ and anti JKb antibody of blood group antibodies in patients with difficult,stating the importance of identification and cross matching blood test for blood transfusion. Methods With using saline、card、polybrene method and coombs method,to detect the blood group serological of patients. Results of serological tests show that the patient ＇s serum didn ＇t agglutinate with Panel cells in saline medium. In the medium of antihuman globulin（ AHG）,the patient＇s serum did agglutinate with E antigen and Jkb antigen positive cell,but not Jkb antigen negative cell among the Panel cells. It＇s determinedthat anti E ＋ and anti Jkb preliminarily. Conclusion by blood grouping the patient was identified as cross matching incompatible induced by anti E ＋ and anti Jkb antibody. So in the clinical work,attention should be paid to the existence of antibodies other than ABO blood group system. Using a variety of antibody methodology,to prevent the detection of omission,which it can improve the safety ofclinical transfusion.

关 键 词：KIDD血型 谱细胞 输血安全 

分 类 号：R457.11[医药卫生—治疗学]