GC-MS分析滴鼻回医香药哈黑里里方的挥发性成分  

Identification of Volatile Chemical Constituents from Hui Formula “Ha Hei Lili” by GC-MS

作　　者：马学琴[1] 王玫[1] 许晓雪[1] 张转转[1] 张万年[1] 

机构地区：[1]宁夏医科大学药学院/宁夏医科大学回药现代化工程技术研究中心,宁夏银川750004

出　　处：《中药材》2015年第10期2176-2179,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技支撑计划课题(2013BAI11B07);宁夏回族自治区科技攻关项目(2012)

摘　　要：目的：分别采用水蒸气蒸馏法（SD）和超临界CO2流体萃取法（SFE）提取滴鼻回医香药哈黑里里方中的挥发性成分,利用GC-MS进行鉴定分析,比较不同提取方法所得成分的差异。方法：GC-MS条件：Rxi-5Sil MS石英毛细管柱（30 m×0.25 mm,0.25μm）;起始温度50℃,保留1 min,以10℃/min升温至120℃维持3 min,以3℃/min升温至200℃维持3 min,以5℃/min升温至290℃维持10 min至完成分析;载气为氦气;柱流量1.0 m L/min;分流比25∶1;进样口温度250℃;EI电离源70 e V;离子源温度230℃;扫描范围m/z 35~500。结果：SD法提取挥发油得率为0.21%,共鉴定出36个化合物,占挥发性成分总量的87.02%;SFE法提取挥发油得率为5.44%,共鉴定出38个化合物,占挥发性成分总量的97.47%。结论：采用SD法和SFE法提取得到的回医香药哈黑里里方挥发性成分有较大差异,为进一步阐明该方药的活性物质基础提供了实验数据。Objective： The volatile components of the Hui formula ＂Ha Hei Lili＂were extracted by steam distillation extraction（ SD） and supercritical CO2 fluid extraction,and the structures were analyzed and identified by GC-MS.Methods： The GC-MS conditions were set as follows： Rxi-5Sil MS quartz capillary column（ 30 m × 0.25 mm,0.25 μm）,the initial temperature of 50 ℃ to keep 1 min,to 10 ℃ / min heating to 120 ℃,maintained 3 min,then to 3 ℃ / min heating to 200 ℃,maintained 3 min,and then to 5 ℃ / min heating to 290 ℃,maintained until completion of analysis; helium as the carrier gas,column flow rate 1.0 m L / min,split ratio 25∶ 1,inlet temperature 250 ℃,EI ionization source 70 e V,ion source temperature 230 ℃,scan range of m / z 35 ~ 500.Results： Yield of volatile oil were 0.21% and 5.44% extracted by SD and SFE methods,respectively; and for SD method,36 kinds of compounds were identified,accounted for 87.02% of total mass of volatile oil; for SFE method,38 kinds of constituents were identified,accounted for 97.47% of total mass of volatile oil.Conclusion： The type of constituents contained in the volatile oil extracted by SD and SFE methods are totally different; and GC-MS can be used to identify the structures and relative content of volatile components,the results of this study can provide an experimental basis for development and utilization of Hui formula ＂Ha Hei Lili＂.

关 键 词：GC-MS 哈黑里里方 化学成分 挥发油 超临界CO_2萃取法 

分 类 号：R284.1[医药卫生—中药学]