辐射诱变水稻雄性不育突变体tda的遗传与细胞学分析  

Genetic and Cytological Analysis of a Rice Male Sterile Mutant tda Derived From Radiation Mutation

作　　者：胡丽芳[1] 苏连水 朱昌兰[1] 彭小松[1] 贺晓鹏[1] 傅军如[1] 陈小荣[1] 欧阳林娟[1] 边建民[1] 贺浩华[1] 

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室/江西省超级稻工程技术研究中心/双季稻现代化生产协同中心,江西南昌330045

出　　处：《核农学报》2015年第12期2253-2258,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31160062);江西省科技厅项目(20122BAB214017);江西省教育厅项目(GJJ12242);江西省重大科技创新研究项目(20133ACF60003)

摘　　要：为了挖掘更多与水稻花药发育相关的基因,利用60Co-γ射线辐照粳稻品种松香早粳,获得1个水稻雄性不育突变体tda,并对其细胞学和遗传学进行分析。结果表明,tda突变体在营养生长期无明显的表型缺陷,花序和花器官能正常发育,但其花药较小且呈白色。组织切片研究发现,tda突变体在小孢子发育时期开始出现异常,绒毡层提前降解,小孢子呈畸形状,随后小孢子萎缩不能形成正常的花粉粒。遗传学分析表明,tda是一个单基因控制的隐性核突变体。该研究结果为TDA基因的克隆、功能分析及水稻花药发育的分子机制奠定了基础。In order to excavate more genes related to rice anther development,one rice male sterility mutant tda（ tapetum degradation anther） was isolated from a japonica rice Songxiangzaogeng through60Co-γ rays irradiation. The characteristic of tda cytological and genetic was investigated. The result showed that,there was no obvious phenotype difference appeared during tda vegetative development stage, the tda anther was small and white although the inflorescence and floral development was normal during the reproductive growth stage. The paraffin sections analysis exhibited that,until meiosis stage,there was no clear difference was observed between tda and wild type anthers. At the microspore stage,tda tapetum displayed aberrant predegradation and the microspore was irregular. Subsequently,the tda microspore was gradually shrinked and could not develop into mature pollen. The genetic analysis found that,tda phenotype was controlled by a single recessive nuclear gene. These results laid a basis for TDA gene cloning,functional analysis and the molecular mechanism research of rice anther development.

关 键 词：辐射诱变 水稻 TDA 花药 绒毡层 小孢子 

分 类 号：S511[农业科学—作物学]