钼胁迫对烟草含钼酶和碳氮代谢关键酶的影响  

Effects on Molybdenum Enzymes and Carbon / Nitrogen Metabolism Enzymes of Tobacco Under Molybdenum Stress

作　　者：武丽[1] 张西仲 唐兴贵 罗倩茜 李余湘 左业华 叶文玲[1] 

机构地区：[1]安徽农业大学农学院,安徽合肥230036 [2]贵州烟草公司黔南州公司,贵州都匀558000

出　　处：《核农学报》2015年第12期2385-2393,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(41301539);安徽省教育厅高校重点基金项目(KJ2013Z085);贵州省黔南州科技局重点科技项目(黔南科合农2013[12])

摘　　要：为了解缺钼对烟草含钼酶和碳氮代谢过程的影响,以南江3号烤烟品种为材料,采用营养液培养,设置施钼(含钼0.20mg·kg-1全营养液)和缺钼(去钼全营养液)2个处理,研究缺钼对烟草发育过程中的含钼酶、碳氮代谢关键酶及其相关代谢产物的影响。结果表明,培养10d时,缺钼处理下,烟草的含钼酶和碳氮代谢关键酶(黄嘌呤脱氢酶、硝酸还原酶、谷氨酰胺合成酶、淀粉酶、磷酸蔗糖合成酶和转化酶)比0d时高,但与施钼处理烟草之间差异不显著;缺钼处理的烟草亚硝酸盐含量显著高于施钼处理烟草。培养20d时,除磷酸蔗糖合成酶的活性提高外,缺钼处理的烟草含钼酶和氮代谢关键酶含量或活性大多显著下降,蔗糖和蛋白质含量均低于施钼处理烟草,亚硝酸盐含量显著高于施钼处理烟草。培养30d时,缺钼处理烟草含钼酶和碳、氮代谢相关酶含量或活性均下降,且显著低于施钼处理烟草;施钼处理烟草则稳中有升,且2个处理烟草的蛋白质和亚硝酸盐含量差异显著。可知,缺钼显著降低烟草中含钼酶和碳氮代谢关键酶活性或含量,抑制了氮代谢,加快了氮代谢向碳代谢的提前转换,使叶片提前落黄。本研究为协调烟草中碳氮代谢提供了准确的生理生化信息。In order to obtain effects on molybdenum enzymes,carbon and nitrogen metabolism process of tobacco,the effects on molybdenum enzymes,carbon / nitrogen metabolism key enzymes and their related metabolites of Nanjiang 3tobacco were studied under molybdenum deficiency stress（ ＋ Mo and-Mo） used by nutrient solution method. Results showed that,when cultured for 10 d,the molybdenum enzymes and carbon / nitrogen metabolism enzymes（ xanthine dehydrogenase,nitrate reductase, glutamine synthetase, amylase, sucrose phosphate synthase and invertase） of molybdenum deficiency tobacco- Mo were higher than those of 0d,but the difference between molybdenum application and molybdenum deficiency tobacco was observed insignificant; when cultured for 20 d,except for sucrose phosphate synthase,other enzymes content or activities were declined,between the two treatments significant and difference was found. And the sucrose and protein content of molybdenum deficiency tobacco were lower than those of molybdenum application tobacco,but the nitrite content was significantly higher than that of molybdenum application tobacco when cultured for 30 d,molybdenum enzymes and carbon / nitrogen metabolism enzymes content or activities were steady for molybdenum application tobacco,but those of molybdenum deficient tobacco decreased. It is suggested that molybdenum deficiency reduces molybdenum enzyme activity or content significantly in tobacco,inhibits the nitrogen metabolism,but promotes the conversion metabolism of nitrogen to carbon and makes the leaves turn yellow in advance. This study provides precise physiological and biochemical information of harmonizing nitrogen / carbon metabolism of tobacco.

关 键 词：缺钼胁迫 烟草 含钼酶 碳氮代谢 代谢产物 

分 类 号：S572[农业科学—烟草工业]