小鼠不同心肌肥厚模型标志性基因表达变化的比较  

Comparison of marker gene expression changes in different mouse models of cardiac hypertrophy

作　　者：阚红卫[1,2] 司文文 尹艳艳[1] 何灿[1] 程杰 汪春彦 张琼光 杨雁[1] 

机构地区：[1]安徽医科大学基础医学院药理教研室,安徽合肥230022 [2]安徽省食品药品审评认证中心,安徽合肥230051 [3]安徽省食品药品检验研究院,安徽合肥230051 [4]湖北省食品药品监督管理局技术审评核查中心,湖北武汉430071

出　　处：《中国药理学通报》2016年第2期274-278,共5页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No 1308085MH144);创新训练项目(No AH201310366005)

摘　　要：目的探索小鼠不同心肌肥厚模型间肥厚性标志基因心房利尿钠肽(ANP)、脑利尿钠肽(BNP)、β-肌球蛋白重链(β-MHC)基因表达的差异。方法取C57BL/6小鼠,采用肾上腹主动脉缩窄(AAC)、动静脉瘘(AVF)和异丙肾上腺素(ISO)分别建立小鼠心肌肥厚模型。造模结束,称取各小鼠体重(BW)、心脏重量(HW)和左室重量(LVW),计算心脏重量指数(HW/BW)和左心室指数(LVW/BW);HE染色观察心肌病理组织形态学变化;免疫组织化学染色观察心肌组织ANP、BNP和β-MHC蛋白表达;采用Real-time PCR检测心肌ANP、BNP和β-MHC mRNA表达。结果与对照组比较,3种模型的HW/BW和LVW/BW升高,光镜下观察各模型小鼠心肌呈现不同程度的肥厚性变化,ANP、BNP和β-MHC在蛋白和mRNA表达水平均不同程度增加;与AAC组比较,AVF和ISO组心肌组织ANP、BNP和β-MHC在蛋白和mRNA表达水平均降低。结论 3种心肌肥厚模型造模成功,AAC模型心肌组织在同时表达肥厚性标志基因ANP、BNP和β-MHC时优于AVF和ISO。Aim To explore the differences in hypertrophic marker genes such as atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP) and β-myosin heavy chain(β-MHC) genes in different models of cardiac hypertrophy.Methods Respectively using renal abdominal aortic coarctation(AAC),arteriovenous fistula(AVF) and isoproterenol(ISO) methods to establish C57BL/6 mouse model of cardiac hypertrophy.After modeling,each mouse' s body weight(BW),heart weight(HW) and left ventricular weight(LVW)were weighed,and the heart weight(HW/BW) and left ventricular index(LVW/BW) were calculated;myocardium by HE staining,pathological morphological changes were observed;myocardium by immunohistochemistry,ANP,BNP and β-MHC protein expression was observed;myocardium by Real-time PCR detection,ANP,BNP and β-MHC mRNA expression was observed.Results Compared with control group,HW/BW and LVW/BW were increased in three models.Through the light microscope,each mouse model showed varying degrees of cardiac hypertrophy.ANP,BNP and β-MHC were increased in the protein and mRNA expression.Compared with AAC group,AVF and ISO groups' myocardial tissue ANP,BNP and β-MHC expression were decreased in the protein and mRNA expression.Conclusions Three cardiac hypertrophy models are successful.Cardiac tissue ANP,BNP and β-MHC expression in AAC model exceeds AVF and ISO model.

关 键 词：小鼠 心肌肥厚 心房利尿钠肽 脑利尿钠肽 Β-肌球蛋白重链 模型 

分 类 号：R-332[医药卫生] R322.11