日粮赖氨酸水平对绿壳蛋种鸡生产性能和血清激素及卵巢FSH和LH受体mRNA表达的影响  

Effects of Dietary Lysine Level on Laying Performance,Serum Hormones and mRNA Expression of Follicle-stimulating Hormone Receptor and Luteinizing Hormone Receptor in Green Shell Laying Breeder Hens

作　　者：丁阳[1] 卜星晨 袁超[1] 严华祥[2] 邹晓庭[1] 

机构地区：[1]浙江大学饲料科学研究所农业部动物营养与饲料科学重点实验室,浙江杭州310058 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《中国畜牧杂志》2016年第3期36-41,共6页Chinese Journal of Animal Science

基　　金：国家蛋鸡产业技术体系建设专项基金(CARS-41-K17);国家科技支撑计划专项基金(2014BAD13B04)

摘　　要：本试验旨在研究日粮赖氨酸水平对绿壳蛋种鸡生产性能、血清激素及卵巢FSH受体(FSHR)、LH受体(LHR)mRNA表达的影响。试验选用30周龄的新杨父母代绿壳蛋鸡720只,随机分成6组,每组5个重复,每个重复24只鸡,分别饲喂赖氨酸水平为0.60%、0.65%、0.70%、0.75%、0.80%和0.85%的日粮。结果表明:0.65%赖氨酸水平组产蛋率显著高于0.85%水平组(P<0.05),0.70%赖氨酸水平组料蛋比显著低于0.60%水平组(P<0.05);血清FSH和LH含量均以0.70%赖氨酸水平组最高(P<0.05);与0.75%和0.85%赖氨酸水平组相比,0.65%水平组卵巢FSHR mRNA的表达量显著提高(P<0.05);血清FSH含量与卵巢FSHR、LHR mRNA表达量呈现较强相关性(r^2>0.85)。二次曲线分析表明,以产蛋率和料蛋比为主要评价指标,绿壳蛋种鸡赖氨酸需要量分别为0.70%和0.73%。综上所述,日粮赖氨酸水平为0.70%~0.73%时能够提高新杨绿壳蛋种鸡(31~43周龄)生产性能、血清促性腺激素含量及卵巢FSHR基因的表达。This study was conducted to investigate the effects of Lysine（Lys） on laying performance,serum hormones and mRNA expression of follicle-stimulating hormone receptor（FSHR） and luteinizinghormone receptor（LHR） in green shell laying breeder hens（31 to 43 wk）.Xinyang green shell laying hens（n=720） 30 wk of age were randomly allocated to 6 treatment groups,each of which included 5 replicates of 24 hens.Lysine was added to basal diet to obtain the Lys level at 0.60%,0.65%,0.70%,0.75%,0.80%and 0.85%,respectively.The results showed that the laying rate in 0.65%Lys group was higher than that in 0.85%Lys group（P〈0.05）.The feed conversion ratio in 0.70%Lys group was the lowest among the groups（P〈0.05）.Serum follicle-stimulating hormone（FSH） and luteinizing hormone（LH） concentrations in 0.70%Lys group were the highest among the groups（P〈0.05）.The FSHR mRNA abundance increased significantly at 0.65%Lys as compared with that in 0.75%and 0.85%Lys groups（P〈0.05）.Serum FSH concentration was closely correlated with the FSHR and LHR mRNA expression（r^20.85）.According to the quadratic regression analysis based on laying rate and feed conversion ratio,the optimal dietary levels of Lys for green shell laying hens（31 to 43 wk） were 0.70%and 0.73%,respectively.The results indicated that 0.70%to 0.73%Lys level could improve laying performance,elevate serum gonadotropic hormone concentration and FSHR mRNA expression in Xingyanggreen shell laying hens（31 to 43 wk）.

关 键 词：赖氨酸 生产性能 血清激素 促卵泡素受体 促黄体素受体 绿壳蛋种鸡 

分 类 号：S831.5[农业科学—畜牧学]