西藏芽孢杆菌XZ7中Amicoumacin类抗生素的分离鉴定与生物活性研究  

Isolation,Identification and Bioactivity of Amicoumacin Group Antibiotics Produced by Bacillus Strain XZ7 Isolated from Tibet

作　　者：刘少伟[1] 夏曼[2] 高强[1] 蒋忠科[1] 胡辛欣[1] 何琪杨[1] 游雪甫[1] 张玉彬[2] 孙承航[1] 

机构地区：[1]中国医学科学院&北京协和医学院医药生物技术研究所,北京100050 [2]中国药科大学生命科学与技术学院,南京210009

出　　处：《天然产物研究与开发》2016年第1期5-10,64,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81402834和81172963);北京市自然科学基金(7154223)

摘　　要：采用UPLC-DAD-MS分析发现西藏当雄土壤来源芽孢杆菌XZ7发酵液中的Amicoumacin类化合物,并利用反相柱层析和高效液相色谱对发现的3个Amicoumacin类化合物进行分离纯化;根据紫外光谱、高分辨质谱和核磁共振波谱数据及文献比对,鉴定3个化合物分别为：Bacilosarcin A（1）、Bacilosarcin B（2）和Bacilosarcin C（3）。抑菌实验结果表明化合物2对表皮葡萄球菌和金黄色葡萄球菌具有较强的抑制活性（MIC=4-16μg/m L）;细胞毒性测试结果表明化合物1和2对HepG2肿瘤细胞具有较高的抑制作用,其中化合物2抑制作用最强,IC_（50）值为2.8μM。同时,化合物2对MCF-7和He La肿瘤细胞也有较强的细胞毒作用,IC（50）值分别为6.2μM和4.0μM。Three Amicoumacin group compounds 1-3 were discovered by UPLC-DAD-MS analysis from the fermentation broth of strain XZ7,a Bacillus sp. isolated from soil of Damxung County in Tibet. The three compounds were isolated by reversed-phase column chromatography and further purified by high performance liquid chromatography. Their chemical structures were elucidated as Bacilosarcin A（ 1）,Bacilosarcin B（ 2） and Bacilosarcin C（ 3） by combinational analysis of spectral data including UV,HR-ESI-MS,NMR and comparison with those of literature. Antimicrobial assay showed compound 2 had the strongest antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus with MIC values of 4-16 μg / m L. Both compound 1 and 2 exhibited cytotoxicity against HepG2 cell line,but compound 2showed stronger cytotoxic activity with IC_（50） value of 2. 8 μM. Meanwhile,IC_（50） values of compound 2 against MCF-7 and He La cell lines were 6. 2 μM and 4. 0 μM,respectively.

关 键 词：Amicoumacin 芽孢杆菌 Bacilosarcin 葡萄球菌 细胞毒活性 

分 类 号：Q939.92[生物学—微生物学]