菟丝子总黄酮对氢化可的松致大鼠少弱精子症的治疗作用及其机制  

Pharmaceutical effects and mechanisms of total flavones from Semen cuscutae on oligoasthenospermia induced by Hydrocortisone in rats

作　　者：孙晶晶[1] 吴秀娟[1] 鲍军[2] 欧阳钢[2] 张鹏翎[2] 徐秋雨[1] 马博[1] 张琪[1] 

机构地区：[1]南京工业大学药学院,江苏南京210009 [2]江苏省省级机关医院,江苏南京210024

出　　处：《华西药学杂志》2016年第1期14-17,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81373478;81370750);江苏省临床医学科技专项(No.BL2013028);江苏省自然科学基金(No.BK20130951)

摘　　要：目的研究菟丝子总黄酮(TFSC)对氢化可的松致大鼠少弱精子症的治疗作用及其可能的作用机制。方法将39只♂Wisatr大鼠随机均分为空白对照组、模型组和TFSC组,每天ip给予模型组和TFSC组大鼠25 mg·kg-1氢化可的松,ip给予空白对照组同体积蒸馏水,连续给药10 d后,从3组中分别随机取出3只大鼠检测脏器指数、生化指标,并采集附睾精子检测数量及活动率。确定造成少弱精子症模型后,每天ig给予TFSC组大鼠100 mg·kg-1TFSC,均ig给予剩余大鼠同体积蒸馏水,治疗15 d后检测大鼠的脏器指数、计量附睾精子数量及活动率,用试剂盒检测血清中睾酮水平、酸性磷酸酯酶(ACP)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)的活性及氧化应激相关指标丙二醛(MDA)、谷胱甘肽(GSH)、总超氧化物歧化酶(T-SOD)的变化,利用Realtime RT-PCR技术检测凋亡基因Fas和Fasl的表达情况。结果 TFSC能明显改善精子质量、促进睾酮分泌、提高睾丸内ACP、LDH、SDH的活性,降低MDA的含量、提高GSH和T-SOD的水平,并下调Fas和Fasl的基因表达。结论TFSC能有效治疗少弱精子症,其机制与抑制氧化应激及下调凋亡基因Fas与Fasl的表达相关。OBJECTIVE To investigate the pharmaceutical effects and the mechanisms of total flavones from Semen cuscutae（ TFSC）on oligoasthenospermia rats induced by Hydrocortisone. METHODS Male Wistar rats were randomly divided into three groups： Blank control group（ vehicle- treated）,model group,and TFSC group. Both model group and TFSC groups were intraperitonealy administrated（ ip） with Hydrocortisone（ 25 mg·kg^- 1） consecutively for the first 10 days in order to establish oligoasthenospermia models. Subsequently,model group was given orally distilled water per day,whereas TFSC group was received TFSC（ 100 mg·kg^- 1） for 15 days. Rats were sacrificed after the last administration,and the weight of organ indexes involving the testis,epididymis and kidney were measured by an electronic balance. The counts and motility of epididymal sperm were also calculated. Different assay kits were used to detect the levels of serum testosterone,the activities of testicular acid phosphatase（ ACP）,lactate dehydrogenase（ LDH） and succinate dehydrogenase（ SDH）. The oxidative stress markers such as malondialdehyde（ MDA）,glutathione（ GSH） and total superoxide dismutase（ T-SOD） were measured by different assay kits according to the manufacturers＇ instructions. The m RNA levels of Fas and Fasl were detected by Realtime RT- PCR. RESULTS The TFSC could significantly improve sperm quality,promote the secretion of testosterone,enhance ACP,LDH,SDH activity,decrease the level of MDA,increase levels of GSH and T- SOD,and down- regulate the expressions of Fas and Fasl. CONCLUSION TFSC can protect the rats from oligoasthenospermia caused by hydrocortisone. The oxidative stress and Fas / Fasl pathways may represent significant therapeutic targets for the prevention or treatment of oligoasthenospermia.

关 键 词：菟丝子总黄酮 氢化可的松 少弱精子症 氧化应激 FAS/FASL 

分 类 号：R96[医药卫生—药理学]