咽炎灵颗粒中药材的薄层色谱鉴别和甘草酸铵的测定  

Identification of Chinese crude by TLC and determination of ammonium glycyrrhetate in Yanyanling granules

作　　者：陈华[1] 于波涛[1] 金伟华[1] 蒲志强[1] 鞠楷 宋宗辉 刘明鲜 范开华[1] 

机构地区：[1]中国人民解放军成都军区总医院药剂科,四川成都610083

出　　处：《华西药学杂志》2016年第1期79-81,共3页West China Journal of Pharmaceutical Sciences

基　　金：军队医疗机构制剂标准提高科研专项课题(No.14ZJZ18-2)

摘　　要：目的建立咽炎灵颗粒的质量控制标准。方法采用薄层色谱法定性鉴别连翘、甘草、玄参药材;采用HPLC法测定甘草酸铵的含量,色谱柱为Agilent HC-C18（250 mm×4.6 mm,5μm）,流动相为乙腈-0.1%磷酸溶液（34∶66）,流速1.0 m L·min^-1,检测波长250 nm,柱温35℃。结果 TLC斑点清晰,分离度较好,专属性强,阴性对照无干扰;1.5-60.0μg·mL^-1甘草酸铵与峰面积的线性关系良好（r=0.9999）,平均回收率为99.52%,RSD=1.87%（n=9）。结论所用方法准确可靠、重复性好,可有效控制咽炎灵颗粒的质量。OBJECTIVE To establish the quality standard for Yanyanling granules. METHODS TLC was used to make qualitative identification of Forsythiae Fructus,Glycyrrhiza Radix et Rhizoma and Scrophulariae Radix,and HPLC was used to do quantitative determination of ammonium glycyrrhetae. The determination was performed on Agilent HC- C18（ 250 mm × 4. 6 mm,5 μm） column with mobile phase consisting of acetonitrile- 0. 1% phosphoric acid（ 34∶66） at the flow rate of 1. 0 m L·min^- 1with the column temperature of 35 ℃ and the detection wavelength of 250 nm. RESULTS The spots of TLC were fairly clear with the good separation,and the blank test showed no interference. The ammonium glycyrrhetate was linear with peak area（ r = 0. 9999） at the range of 1. 5- 60. 0 μg·mL^-1,and its average recovery rate was 99. 52% and RSD was 1. 87%（ n = 9）. CONCLUSION The method is accurate,reliable and reproducible,and can be used for the quality control of Yanyanling granules.

关 键 词：咽炎灵颗粒 质量标准 薄层色谱法 高效液相色谱法 甘草酸铵 

分 类 号：R917[医药卫生—药物分析学]