高压电烧伤对SD大鼠血栓调节蛋白、蛋白C、蛋白S及D二聚体的早期影响  

The influence of high-voltage electrical burn on the thrombomodulin, protein C, protein S, and D-Dimer in SD rats

作　　者：刘泽世[1] 呼瑞 刘二军[3] 周谨[3] 

机构地区：[1]西安交通大学医学院第二附属医院检验科,710003 [2]陕西省西北妇女儿童医院,西安市710003 [3]河北医科大学第一医院,石家庄市050031

出　　处：《实用医学杂志》2016年第1期37-40,共4页The Journal of Practical Medicine

基　　金：河北省医学科学研究重点课题计划(编号:20130563)

摘　　要：目的:探讨高压电烧伤对SD大鼠外周血血栓调节蛋白(thronlbonlodulin,TM)、蛋白C(protein C,PC)、蛋白S(protein S,PS)及D二聚体(D-Dimer,D-D)的影响。方法:将120只SD大鼠按随机数字表法分为假高压电烧伤组(简称假伤组)、高压电烧伤组(简称电伤组),每组60只。2组致伤前15 min按随机数字表法各取10只大鼠,心脏采血分离血浆。电伤组余下50只大鼠用调压器和实验变压器造成高压电烧伤,假伤组余下50只大鼠按相同装置不通电模拟致伤。伤后5 min和1、2、4、8 h,每组SD大鼠按随机数字表法各取10只大鼠,心脏采血分离血浆。测定各时相点血清中TM、PC、PS及血浆中D-D的变化。对数据行析因设计方差分析、LSD检验。结果:电伤组SD大鼠的TM浓度在伤后5 min至8 h明显高于假伤组(均P<0.05):除伤前15 min(P>0.05)外,电伤组大鼠其余各时相点PC、PS浓度均低于假伤组(均P<0.05):D-D浓度在伤后8 h达到最高值[(173.05±4.08)ng/mL]。结论:高压电烧伤早期,SD大鼠TM、D-D的浓度升高,PC、PS的浓度降低,血管内皮细胞的完整性被破坏,凝血功能受到严重影响。Objective To investigate the influence of high-voltage electrical burn on the throm-bomodulin（TM）,protein C（PC）,protein S（PS） and D-Dimer（D-D） in SD rats.Methods One hundred and twenty healthy SD rats were divided into the fake high-voltage electrical burn groups（FHEB）,high-voltage electrical burn groups（HEB） according to the random number table,with 60 rats in each group.Ten rats were taken from each group at 15 minutes before injury.Plasma were collected from heart blood.Fifty SD rats of HEB group with voltage regulator and experimental transformer.The remaining fifty SD rats of FHEB group were sham injured with the same devices without electric current.At 5 minutes and 1,2,4,8 hour（s） post injury,10 rats of every group were randomly chosen at each time point for observation of the concentrations of TM,PC,PS and D-D.Plasma were collected from heart blood.Data were processed with analysis of variance of factorial design and LSD test.Results Compared with the FHEB group,the concentration of TM from 5 minutes to 8 hours post injury in HEB group was higher significantly（P〈0.05）.Exception of the concentrations of PC and PS at 15 minutes before injury,the concentrations of PC and PS were lower than those of FHEB group（P〈0.05）.The concentration of D-D in HEB group peaked at 8 hours post injury in（173.05 ± 4.08） ng/mL.Conclusion High-voltage electrical burn at early stage can increase the concentrations of TM,D-D,as well as decrease the concentrations of PC and PS,which are not only causing the vascular endothelium damage but also possessing serious effect on the thromboplastin function of SD rats.

关 键 词：高压电烧伤 血栓调节蛋白 蛋白C 蛋白S D二聚体 

分 类 号：R647[医药卫生—外科学]