水飞蓟素逆转人乳腺癌耐药细胞株MCF-7/ADM耐药性实验研究  

Reversal Effect of Silymarin on Drug Resistant Breast Cancer Cell MCF-7/ADM

作　　者：陆一丹[1] 徐良额 裘嘉琪 毛丹漪[1] 刘晓谷[1] 王大维[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中西医结合杂志》2016年第2期117-119,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省大学生科技创新活动计划(新苗人才计划)项目(No.2014R410038);浙江省中医药科技计划项目(No.2016ZB030)

摘　　要：目的观察水飞蓟素(Silymarin,Sily)对人乳腺癌耐药细胞株MCF-7/ADM的逆转耐药作用。方法以CCK-8法测定阿霉素(Adm)对人乳腺癌敏感细胞株MCF-7/S和耐药细胞株MCF-7/ADM的毒性作用,计算出耐药倍数。以无细胞毒性的Sily(10μg/m L)作为逆转耐药剂,联合Adm观察其对耐药细胞株MCF-7/ADM的逆转耐药作用,计算得逆转倍数。结果 (1)Adm对MCF-7/S和MCF-7/ADM的半数抑制浓度(IC50)分别为1.773μg/m L和43.812μg/m L,耐药倍数为24.7倍。(2)Sily能够增强ADM对MCF-7/ADM的细胞毒作用。以10μg/m L(抑制率为2.0%)的Sily联合Adm作用于MCF-7/ADM 48h后,耐药细胞株的IC50降至7.798μg/m L,逆转倍数为5.6倍(P<0.01)。结论Sily能够逆转人乳腺癌耐药细胞株MCF-7/ADM的耐药性。Objective To investigate the effect of silymarin on drug resistance of breast cancer cell line MCF-7/ADM. Methods The toxicant effect of adriamycin to MCF-7/S（sensitive strain） and MCF-7/ADM（drug-resistant strain） was measured by CCK-8 in vitrofor resistant index. Silymarin was screened in advance and the dose of 10μg/ml was nontoxicant to MCF- 7/ADM, then MCF- 7/ADM was treated with this dose of silymarin（10μg/ml） to observe the reversal effect of silymarin. Results IC50 of adriamycin to MCF-7/S and MCF-7/ADM were 1.773 μg/ml and 43.812μg/ml, respectively. Drug-resistant index of MCF-7/ADM was 24.7. Silymarin increased cell toxic effect of adriamycin. After treated with 10μg/mlsilymarin（inhibition rate was 2.0%） combining with adriamycin for48 h, IC50 of adriamycine declined to 7.798μg/ml, and the reversal index was 5.6（P〈0.01）. Conclusion Silymarin could partly reverse drug-resistance of MCF- 7/ADMto adriamycinein vitro.

关 键 词：乳腺癌 耐药细胞株 水飞蓟素 逆转耐药 

分 类 号：R285[医药卫生—中药学]